5EM2

Crystal structure of the Erb1-Ytm1 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Ribosome biogenesis protein ERB1	polymer	388	43174.3	2	UniProt (G0SCK6) Pfam (PF00400) In PDB	Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)	Eukaryotic ribosome biogenesis protein 1
2	B, D	Ribosome biogenesis protein YTM1	polymer	499	53326.6	2	UniProt (G0SFB5) Pfam (PF08154) Pfam (PF00400) In PDB	Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
3	A, C, D	1,2-ETHANEDIOL	non-polymer		62.1	11	Chemie (EDO)
4	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5		water	water		18.0	183	Chemie (HOH)

Sequence modifications

A, C: 423 - 801 (UniProt: G0SCK6)

PDB	External Database	Details
Met 414	-	initiating methionine
Ala 415	-	expression tag
His 416	-	expression tag
His 417	-	expression tag
His 418	-	expression tag
His 419	-	expression tag
His 420	-	expression tag
His 421	-	expression tag
Met 422	-	expression tag

B, D: 1 - 495 (UniProt: G0SFB5)

PDB	External Database	Details
Gly -3	-	expression tag
Gly -2	-	expression tag
Ala -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	193001.9
Non-Polymers*	Number of molecules	12
	Total formula weight	707.1
All*	Total formula weight	193708.9

*Water molecules are not included.