5EM2

Crystal structure of the Erb1-Ytm1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-13
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.8726
Spacegroup name	P 1 21 1
Unit cell lengths	91.662, 81.353, 141.695
Unit cell angles	90.00, 100.23, 90.00

Refinement procedure

Resolution	48.609 - 2.670
R-factor	0.2225
Rwork	0.221
R-free	0.25090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.917
Data reduction software	XDS
Data scaling software	Aimless (0.3.11)
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.610	48.610	2.740
High resolution limit [Å]	2.670	11.640	2.670
Rmerge	0.163	0.037	1.176
Rpim	0.077	0.018	0.567
Total number of observations	306061	3788	23266
Number of reflections	58449
<I/σ(I)>	10.6	36.5	1.5
Completeness [%]	99.8	98.5	100
Redundancy	5.2	5.1	5.2
CC(1/2)	0.992	0.999	0.529

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	20-28% ethylene glycol