5ELV

Crystal structure of the GluA2 ligand-binding domain (S1S2J-L504-N775) in complex with glutamate and BPAM-521 at 1.92 A resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Glutamate receptor 2,Glutamate receptor 2	polymer	264	29301.7	2	UniProt (P19491) Pfam (PF10613) Pfam (PF00060) In PDB	Rattus norvegicus (Rat)	GluR-2,AMPA-selective glutamate receptor 2,GluR-B,GluR-K2,Glutamate receptor ionotropic,AMPA 2,GluA2
2	A, B	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
3	A, B	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
4	A, B	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
5	A, B	ACETATE ION	non-polymer		59.0	9	Chemie (ACT)
6	B, A	4-Cyclopropyl-3,4-dihydro-7-hydroxy-2H-1,2,4-benzothiadiazine 1,1-dioxide	non-polymer		240.3	2	Chemie (5PX)
7	B, A	GLUTAMIC ACID	non-polymer		147.1	2	Chemie (GLU)
8	B	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
9		water	water		18.0	443	Chemie (HOH)

Sequence modifications

A, B: 3 - 117 (UniProt: P19491)

PDB	External Database	Details
Gly 1	-	expression tag
Ala 2	-	expression tag
Tyr 94	Leu 504	engineered mutation

A, B: 120 - 264 (UniProt: P19491)

PDB	External Database	Details
Gly 118	-	linker
Thr 119	-	linker
Ser 242	Asn 775	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	58603.5
Non-Polymers*	Number of molecules	26
	Total formula weight	2377.4
All*	Total formula weight	60980.9

*Water molecules are not included.