5EJI
Crystal structure of NAD kinase W78F mutant from Listeria monocytogenes in complex with NADP/Mn++/PPi
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | NAD kinase 1 | polymer | 272 | 31006.2 | 1 | UniProt (Q8Y8D7) Pfam (PF01513) Pfam (PF20143) | Listeria monocytogenes | ATP-dependent NAD kinase |
| 2 | B (A) | NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 743.4 | 1 | Chemie (NAP) | |||
| 3 | C (A) | MANGANESE (II) ION | non-polymer | 54.9 | 1 | Chemie (MN) | |||
| 4 | D (A) | PYROPHOSPHATE 2- | non-polymer | 176.0 | 1 | Chemie (POP) | |||
| 5 | E (A) | CITRIC ACID | non-polymer | 192.1 | 1 | Chemie (CIT) | |||
| 6 | F (A) | water | water | 18.0 | 34 | Chemie (HOH) |
Sequence modifications
A: 1 - 264 (UniProt: Q8Y8D7)
| PDB | External Database | Details |
|---|---|---|
| Phe 78 | Trp 78 | engineered mutation |
| Leu 265 | - | expression tag |
| Glu 266 | - | expression tag |
| His 267 | - | expression tag |
| His 268 | - | expression tag |
| His 269 | - | expression tag |
| His 270 | - | expression tag |
| His 271 | - | expression tag |
| His 272 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 31006.2 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1166.4 | |
| All* | Total formula weight | 32172.7 |
