5EJI

Crystal structure of NAD kinase W78F mutant from Listeria monocytogenes in complex with NADP/Mn++/PPi

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	NAD kinase 1	polymer	272	31006.2	1	UniProt (Q8Y8D7) Pfam (PF01513) Pfam (PF20143) In PDB	Listeria monocytogenes	ATP-dependent NAD kinase
2	A	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		743.4	1	Chemie (NAP)
3	A	MANGANESE (II) ION	non-polymer		54.9	1	Chemie (MN)
4	A	PYROPHOSPHATE 2-	non-polymer		176.0	1	Chemie (POP)
5	A	CITRIC ACID	non-polymer		192.1	1	Chemie (CIT)
6		water	water		18.0	34	Chemie (HOH)

Sequence modifications

A: 1 - 264 (UniProt: Q8Y8D7)

PDB	External Database	Details
Phe 78	Trp 78	engineered mutation
Leu 265	-	expression tag
Glu 266	-	expression tag
His 267	-	expression tag
His 268	-	expression tag
His 269	-	expression tag
His 270	-	expression tag
His 271	-	expression tag
His 272	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	31006.2
Non-Polymers*	Number of molecules	4
	Total formula weight	1166.4
All*	Total formula weight	32172.7

*Water molecules are not included.