5EGE

Structure of ENPP6, a choline-specific glycerophosphodiester-phosphodiesterase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Ectonucleotide pyrophosphatase/phosphodiesterase family member 6	polymer	429	49598.7	4	UniProt (Q8BGN3) Pfam (PF01663)	Mus musculus (Mouse)	NPP-6,Choline-specific glycerophosphodiester phosphodiesterase,Glycerophosphocholine cholinephosphodiesterase,GPC-Cpde
2	E, G, I, K (E, G, I, K)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[beta-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	4	In PDB GlyTouCan (G28454KX)
3	F, H, J, L (F, H, J, L)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	4	In PDB GlyTouCan (G42666HT)
4	DA, M, N, S, T... (D, A, B, C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	7	Chemie (NAG)
5	AA, EA, FA, O, P... (C, D, A, B)	ZINC ION	non-polymer		65.4	8	Chemie (ZN)
6	BA, CA, GA, Q, R... (C, D, A, B)	1,2-ETHANEDIOL	non-polymer		62.1	6	Chemie (EDO)
7	HA, IA, JA, KA (A, B, C, D)	water	water		18.0	2048	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 421 (UniProt: Q8BGN3)

PDB	External Database	Details
Ala 393	Cys 393	engineered mutation
Ser 412	Cys 412	engineered mutation
Ser 422	-	expression tag
Arg 423	-	expression tag
Glu 424	-	expression tag
Asn 425	-	expression tag
Leu 426	-	expression tag
Tyr 427	-	expression tag
Phe 428	-	expression tag
Gln 429	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	198394.7
Branched	Number of molecules	8
	Total formula weight	3979.8
Non-Polymers*	Number of molecules	21
	Total formula weight	2444.1
All*	Total formula weight	204818.6

*Water molecules are not included.