5EGE
Structure of ENPP6, a choline-specific glycerophosphodiester-phosphodiesterase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-09 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.282 |
| Spacegroup name | P 1 |
| Unit cell lengths | 64.290, 78.240, 103.640 |
| Unit cell angles | 90.04, 89.98, 114.61 |
Refinement procedure
| Resolution | 41.896 - 2.000 |
| R-factor | 0.1689 |
| Rwork | 0.167 |
| R-free | 0.20570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.890 |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10_2155: 000)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 120105 |
| <I/σ(I)> | 6.11 |
| Completeness [%] | 97.0 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 293 | ammonium chloride, sodium acetate pH, PEG 6000 |
