5EG3

Crystal Structure of the Activated FGF Receptor 2 (FGFR2) Kinase Domain in complex with the cSH2 domain of Phospholipase C gamma (PLCgamma)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Fibroblast growth factor receptor 2	polymer	334	38155.7	1	UniProt (P21802) Pfam (PF07714)	Homo sapiens (Human)	FGFR-2,K-sam,KGFR,Keratinocyte growth factor receptor
2	B (B)	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1	polymer	114	13310.1	1	UniProt (P10686) Pfam (PF00017)	Rattus norvegicus (Rat)	Phosphoinositide phospholipase C-gamma-1,Phospholipase C-gamma-1,PLC-gamma-1
3	C (A)	PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER	non-polymer		505.2	1	Chemie (ACP)
4	D, E (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	F, G (A, B)	water	water		18.0	55	Chemie (HOH)

Sequence modifications

A: 458 - 778 (UniProt: P21802)

PDB	External Database	Details
Met 445	-	initiating methionine
Gly 446	-	expression tag
Ser 447	-	expression tag
Ser 448	-	expression tag
His 449	-	expression tag
His 450	-	expression tag
His 451	-	expression tag
His 452	-	expression tag
His 453	-	expression tag
His 454	-	expression tag
Ser 455	-	expression tag
Gln 456	-	expression tag
Asp 457	-	expression tag
Phe 466	Tyr 466	engineered mutation
Ala 491	Cys 491	engineered mutation
Ala 565	Glu 565	engineered mutation
Leu 586	Tyr 586	engineered mutation
Pro 588	Tyr 588	engineered mutation
Phe 656	Tyr 656	engineered mutation
Phe 657	Tyr 657	engineered mutation
Glu 659	Lys 659	engineered mutation

B: 661 - 773 (UniProt: P10686)

PDB	External Database	Details
Met 660	-	initiating methionine

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	51465.8
Non-Polymers*	Number of molecules	3
	Total formula weight	553.8
All*	Total formula weight	52019.6

*Water molecules are not included.