5EG3
Crystal Structure of the Activated FGF Receptor 2 (FGFR2) Kinase Domain in complex with the cSH2 domain of Phospholipase C gamma (PLCgamma)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-20 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97907 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 79.609, 53.231, 127.660 |
Unit cell angles | 90.00, 100.11, 90.00 |
Refinement procedure
Resolution | 37.859 - 2.606 |
R-factor | 0.1907 |
Rwork | 0.185 |
R-free | 0.23730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pvy 2ple |
RMSD bond length | 0.009 |
RMSD bond angle | 1.210 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.859 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.256 | |
Number of reflections | 15981 | |
<I/σ(I)> | 27.9 | 5.9 |
Completeness [%] | 98.2 | 93.5 |
Redundancy | 6.6 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 25 mM HEPES (pH 7.5), PEG20000 (12% 18%) and 2% (w/v) Benzamidine hydrochloride |