5EDV

Structure of the HOIP-RBR/UbcH5B~ubiquitin transfer complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	E3 ubiquitin-protein ligase RNF31	polymer	380	43528.7	2	UniProt (Q96EP0) Pfam (PF22191) Pfam (PF18091)	Homo sapiens (Human)	HOIL-1-interacting protein,HOIP,RING finger protein 31,Zinc in-between-RING-finger ubiquitin-associated domain protein
2	C, D, I (C, D, I)	Ubiquitin-conjugating enzyme E2 D2	polymer	149	16931.4	3	UniProt (P62837) Pfam (PF00179)	Homo sapiens (Human)	(E3-independent) E2 ubiquitin-conjugating enzyme D2,E2 ubiquitin-conjugating enzyme D2,Ubiquitin carrier protein D2,Ubiquitin-conjugating enzyme E2(17)KB 2,Ubiquitin-conjugating enzyme E2-17 kDa 2,Ubiquitin-protein ligase D2,p53-regulated ubiquitin-conjugating enzyme 1
3	E, F, G, H (E, F, G, H)	Polyubiquitin-B	polymer	76	8576.8	4	UniProt (P0CG47) Pfam (PF00240)	Homo sapiens (Human)
4	J, K, L, M, N... (A, B)	ZINC ION	non-polymer		65.4	16	Chemie (ZN)

Sequence modifications

A, B: 696 - 1072 (UniProt: Q96EP0)

PDB	External Database	Details
Gly 693	-	expression tag
Pro 694	-	expression tag
Gly 695	-	expression tag

C, D, I: 2 - 147 (UniProt: P62837)

PDB	External Database	Details
Gly -1	-	expression tag
Pro 0	-	expression tag
Gly 1	-	expression tag
Arg 22	Ser 22	engineered mutation
Lys 85	Cys 85	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	172158.9
Non-Polymers*	Number of molecules	16
	Total formula weight	1046.5
All*	Total formula weight	173205.4

*Water molecules are not included.