5EDV
Structure of the HOIP-RBR/UbcH5B~ubiquitin transfer complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.282 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 104.820, 75.740, 120.960 |
| Unit cell angles | 90.00, 95.56, 90.00 |
Refinement procedure
| Resolution | 29.690 - 3.480 |
| R-factor | 0.25137 |
| Rwork | 0.249 |
| R-free | 0.30260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4LJP chains A and B; 3A33 chain A and 2CT7 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.465 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.690 | 3.720 |
| High resolution limit [Å] | 3.480 | 3.480 |
| Rmerge | 0.093 | 0.875 |
| Number of reflections | 22930 | |
| <I/σ(I)> | 9.9 | 1.6 |
| Completeness [%] | 98.6 | 96.1 |
| Redundancy | 6.5 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 285 | 0.06M Tris, 0.04M Bicine, 0.02 M 1,6-Hexanediol, 0.02 M 1-Butanol, 0.02 M 1,2- Propanediol (racemic), 0.02 M 2-Propanol, 0.02 M 1,4-Butanediol, 0.02 M 1,3-Propanediol, 20% PEG550MME, 10% PEG20K, 8% glycerol |
