5ECD

Structure of the Shigella flexneri VapC mutant D98N crystal form 1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	tRNA(fMet)-specific endonuclease VapC	polymer	138	15664.0	2	UniProt (O06662) Pfam (PF01850)	Shigella flexneri	RNase VapC,Toxin VapC
2	C, D (A, B)	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	2	Chemie (EPE)
3	E, F (A, B)	water	water		18.0	270	Chemie (HOH)

Sequence modifications

A, B: 2 - 132 (UniProt: O06662)

PDB	External Database	Details
Met -5	-	initiating methionine
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Asn 98	Asp 98	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	31327.9
Non-Polymers*	Number of molecules	2
	Total formula weight	476.6
All*	Total formula weight	31804.6

*Water molecules are not included.