5E4R

Crystal structure of domain-duplicated synthetic class II ketol-acid reductoisomerase 2Ia_KARI-DD

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Ketol-acid reductoisomerase	polymer	490	55261.9	1	UniProt (E0SRA9) Pfam (PF07991) Pfam (PF01450) In PDB	Ignisphaera aggregans (strain DSM 17230 / JCM 13409 / AQ1.S1)	Acetohydroxy-acid isomeroreductase,Alpha-keto-beta-hydroxylacyl reductoisomerase
2	A	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		743.4	1	Chemie (NAP)
3	A	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	A	oxo(propan-2-ylamino)acetic acid	non-polymer		131.1	1	Chemie (40E)
5	A	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
6	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
7		water	water		18.0	237	Chemie (HOH)

Sequence modifications

A: 1 - 335 (UniProt: E0SRA9)
A: 336 - 482 (UniProt: E0SRA9)

PDB	External Database	Details
Leu 483	-	expression tag
Glu 484	-	expression tag
His 485	-	expression tag
His 486	-	expression tag
His 487	-	expression tag
His 488	-	expression tag
His 489	-	expression tag
His 490	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	55261.9
Non-Polymers*	Number of molecules	6
	Total formula weight	1077.3
All*	Total formula weight	56339.2

*Water molecules are not included.