5E4R

Crystal structure of domain-duplicated synthetic class II ketol-acid reductoisomerase 2Ia_KARI-DD

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004455	molecular_function	ketol-acid reductoisomerase activity
A	0009082	biological_process	branched-chain amino acid biosynthetic process
A	0016491	molecular_function	oxidoreductase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	35
Details	binding site for residue NAP A 501

Chain	Residue
A	GLY24
A	PRO82
A	ASP83
A	VAL85
A	GLN86
A	ALA107
A	HIS108
A	SER132
A	PRO133
A	GLY134
A	ALA397
A	TYR25
A	VAL398
A	SER399
A	40E504
A	EDO505
A	HOH613
A	HOH641
A	HOH670
A	HOH672
A	HOH686
A	HOH704
A	GLY26
A	HOH709
A	HOH712
A	HOH728
A	HOH751
A	HOH775
A	HOH802
A	SER27
A	GLN28
A	LEU47
A	SER53
A	LEU80
A	VAL81

---

site_id	AC2
Number of Residues	6
Details	binding site for residue MG A 502

Chain	Residue
A	ASP191
A	GLU195
A	MG503
A	40E504
A	HOH683
A	HOH775

---

site_id	AC3
Number of Residues	7
Details	binding site for residue MG A 503

Chain	Residue
A	ASP191
A	MG502
A	40E504
A	HOH601
A	HOH627
A	HOH638
A	HOH675

---

site_id	AC4
Number of Residues	15
Details	binding site for residue 40E A 504

Chain	Residue
A	PRO133
A	ASP191
A	GLU195
A	GLU378
A	VAL398
A	SER399
A	ALA402
A	NAP501
A	MG502
A	MG503
A	HOH601
A	HOH638
A	HOH675
A	HOH683
A	HOH775

---

site_id	AC5
Number of Residues	9
Details	binding site for residue EDO A 505

Chain	Residue
A	LEU23
A	GLY46
A	LEU47
A	GLU48
A	THR67
A	VAL81
A	LEU89
A	NAP501
A	HOH728

---

site_id	AC6
Number of Residues	5
Details	binding site for residue GOL A 506

Chain	Residue
A	ASP7
A	LEU12
A	LYS170
A	ARG176
A	HOH676

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: ACT_SITE => ECO:0000255|HAMAP-Rule:MF_00435

Chain	Residue	Details
A	HIS108

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00435, ECO:0000269|PubMed:25849365, ECO:0007744|PDB:4XDZ, ECO:0007744|PDB:5E4R

Chain	Residue	Details
A	TYR25
A	SER53
A	ASP83
A	GLY134

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:25849365, ECO:0007744|PDB:4XDZ, ECO:0007744|PDB:5E4R

Chain	Residue	Details
A	ARG49

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00435

Chain	Residue	Details
A	ASP191
A	GLU227
A	GLU231
A	SER252

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00435, ECO:0000269|PubMed:25849365

Chain	Residue	Details
A	GLU195

---