5DZF
Crystal Structure of the catalytic nucleophile mutant of VvEG16 in complex with a mixed-linkage glucan octasaccharide
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | endo-glucanase | polymer | 208 | 23296.9 | 2 | UniProt (F6I323) Pfam (PF00722) | Vitis vinifera (Grape) | |
| 2 | C (C) | beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 666.6 | 1 | In PDB GlyTouCan (G15359HQ) | |||
| 3 | D (D) | beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 1315.1 | 1 | In PDB GlyTouCan (G57485LV) | |||
| 4 | E, F, H (A, B) | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
| 5 | G (B) | beta-D-glucopyranose | non-polymer | 180.2 | 1 | Chemie (BGC) | |||
| 6 | I, J (A, B) | water | water | 18.0 | 447 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 208 (UniProt: F6I323)
| PDB | External Database | Details |
|---|---|---|
| Gly 1 | - | expression tag |
| Ala 89 | Glu 88 | engineered mutation |
| - | Val 151 | deletion |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 46593.9 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 1981.7 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 468.3 | |
| All* | Total formula weight | 49043.9 |
