5DNE
Crystal structure of the Asn-bound guinea pig L-asparaginase 1 catalytic domain active site mutant K188M
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | L-asparaginase | polymer | 588 | 63386.8 | 4 | UniProt (H0W0T5) Pfam (PF00710) Pfam (PF17763) Pfam (PF12796) | Cavia porcellus (Guinea pig) | |
| 2 | E, H, K, P (A, B, C, D) | ASPARAGINE | non-polymer | 132.1 | 4 | Chemie (ASN) | |||
| 3 | F, G, I, J, L... (A, B, C, D) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 9 | Chemie (EDO) | |||
| 4 | R, S, T, U (A, B, C, D) | water | water | 18.0 | 320 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 565 (UniProt: H0W0T5)
| PDB | External Database | Details |
|---|---|---|
| Met -22 | - | initiating methionine |
| Gly -21 | - | expression tag |
| Ser -20 | - | expression tag |
| Ser -19 | - | expression tag |
| His -18 | - | expression tag |
| His -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| Ser -12 | - | expression tag |
| Ser -11 | - | expression tag |
| Gly -10 | - | expression tag |
| Gly -9 | - | expression tag |
| Asn -8 | - | expression tag |
| Glu -7 | - | expression tag |
| Asn -6 | - | expression tag |
| Leu -5 | - | expression tag |
| Tyr -4 | - | expression tag |
| Phe -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Gly -1 | - | expression tag |
| His 0 | - | expression tag |
| Met 188 | Lys 188 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 253547.0 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 1087.1 | |
| All* | Total formula weight | 254634.1 |
