5DNE
Crystal structure of the Asn-bound guinea pig L-asparaginase 1 catalytic domain active site mutant K188M
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 123.910, 154.790, 157.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.390 |
| R-factor | 0.19574 |
| Rwork | 0.194 |
| R-free | 0.23709 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r8l |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.319 |
| Data reduction software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.530 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Number of reflections | 57320 | |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 95.5 | 93.8 |
| Redundancy | 3.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M HEPES pH 7.0, 12-15% PEG 4000 |
