5D80

Crystal Structure of Yeast V1-ATPase in the Autoinhibited Form

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, a, b...	V-type proton ATPase catalytic subunit A	polymer	617	67796.5	6	UniProt (P17255) Pfam (PF02874) Pfam (PF16886) Pfam (PF00006) In PDB	Saccharomyces cerevisiae (Baker's yeast)	V-ATPase subunit A,Vacuolar proton pump subunit A
2	D, E, F, d, e...	V-type proton ATPase subunit B	polymer	517	57815.0	6	UniProt (P16140) Pfam (PF02874) Pfam (PF00006) In PDB	Saccharomyces cerevisiae (Baker's yeast)	V-ATPase subunit B,V-ATPase 57 kDa subunit,Vacuolar proton pump subunit B
3	H, h	V-type proton ATPase subunit H	polymer	478	54482.6	2	UniProt (P41807) Pfam (PF03224) Pfam (PF11698) In PDB	Saccharomyces cerevisiae (Baker's yeast)	V-ATPase subunit H,V-ATPase 54 kDa subunit,Vacuolar proton pump subunit H
4	J, L, N, j, l...	V-type proton ATPase subunit G	polymer	122	13735.7	6	UniProt (P48836) Pfam (PF03179) In PDB	Saccharomyces cerevisiae (Baker's yeast)	V-ATPase subunit G,V-ATPase 13 kDa subunit,Vacuolar proton pump subunit G
5	I, K, M, i, k...	V-type proton ATPase subunit E	polymer	233	26508.4	6	UniProt (P22203) Pfam (PF01991) In PDB	Saccharomyces cerevisiae (Baker's yeast)	V-ATPase subunit E,V-ATPase 27 kDa subunit,Vacuolar proton pump subunit E
6	G, g	V-type proton ATPase subunit D	polymer	256	29235.0	2	UniProt (P32610) Pfam (PF01813) In PDB	Saccharomyces cerevisiae (Baker's yeast)	V-ATPase subunit D,Vacuolar proton pump subunit D
7	o, O	V-type proton ATPase subunit F	polymer	118	13479.2	2	UniProt (P39111) Pfam (PF01990) In PDB	Saccharomyces cerevisiae (Baker's yeast)	V-ATPase subunit F,V-ATPase 14 kDa subunit,Vacuolar proton pump subunit F

Sequence modifications

A, B, C, a, b, c: 1 - 283 (UniProt: P17255)
A, B, C, a, b, c: 284 - 617 (UniProt: P17255)
J, L, N, j, l, n: 2 - 114 (UniProt: P48836)

PDB	External Database	Details
Met -7	-	initiating methionine
Asp -6	-	expression tag
Tyr -5	-	expression tag
Lys -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Asp 0	-	expression tag
Lys 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	30
	Total formula weight	1189527.2
All*	Total formula weight	1189527.2

*Water molecules are not included.