5D80

Crystal Structure of Yeast V1-ATPase in the Autoinhibited Form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-10
Detector	ADSC QUANTUM 210
Wavelength(s)	0.6279
Spacegroup name	C 1 2 1
Unit cell lengths	468.020, 159.650, 248.270
Unit cell angles	90.00, 113.75, 90.00

Refinement procedure

Resolution	39.720 - 6.202
R-factor	0.2573
Rwork	0.255
R-free	0.30180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5bw9
RMSD bond length	0.002
RMSD bond angle	0.623
Data scaling software	Aimless (0.2.17)
Phasing software	PHASER
Refinement software	PHENIX (dev-1957)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.720	39.720	6.340
High resolution limit [Å]	6.010	19.020	6.010
Rmerge	0.258	0.225	4.349
Rpim	0.083	0.085	1.367
Total number of observations	463370	11036	65486
Number of reflections	41729
<I/σ(I)>	11	37.5	0.6
Completeness [%]	99.3	89.2	97.5
Redundancy	11.1	8.9	11
CC(1/2)	0.981	0.928	0.232

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	8.25% PEG 8000, 0.25 M Ammonium Sulfate, 0.1 M HEPES pH 7.5, 0.05 M Strontium Chloride