5D46

Structural Basis for a New Templated Activity by Terminal Deoxynucleotidyl Transferase: Implications for V(D)J Recombination

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Terminal deoxynucleotidyltransferase	polymer	400	45704.0	1	UniProt (Q3UZ80) Pfam (PF14716) Pfam (PF10391) Pfam (PF01909) Pfam (PF14791) UniProt (by SIFTS) (P09838)	Mus musculus (Mouse)
2	B, E (B, E)	DNA (5'-D(*AP*AP*AP*AP*AP*A)-3')	polymer	6	1810.3	2		synthetic construct
3	C, D (C, D)	DNA (5'-D(*TP*TP*TP*TP*TP*GP*C)-3')	polymer	7	2094.4	2		synthetic construct
4	F (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	G (A)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
6	H (A)	PYROPHOSPHATE 2-	non-polymer		176.0	1	Chemie (POP)
7	I, J (A)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
8	K, L, M (A)	ACETATE ION	non-polymer		59.0	3	Chemie (ACT)
9	N, O, P, Q, R (A, B, C, D, E)	water	water		18.0	104	Chemie (HOH)

Sequence modifications

A: 132 - 510 (UniProt: Q3UZ80)

PDB	External Database	Details
Met 111	-	initiating methionine
Gly 112	-	expression tag
Ser 113	-	expression tag
Ser 114	-	expression tag
His 115	-	expression tag
His 116	-	expression tag
His 117	-	expression tag
His 118	-	expression tag
His 119	-	expression tag
His 120	-	expression tag
Ser 121	-	expression tag
Ser 122	-	expression tag
Gly 123	-	expression tag
Leu 124	-	expression tag
Val 125	-	expression tag
Pro 126	-	expression tag
Arg 127	-	expression tag
Gly 128	-	expression tag
Ser 129	-	expression tag
His 130	-	expression tag
Met 131	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	53513.3
Non-Polymers*	Number of molecules	8
	Total formula weight	592.5
All*	Total formula weight	54105.8

*Water molecules are not included.