5D46
Structural Basis for a New Templated Activity by Terminal Deoxynucleotidyl Transferase: Implications for V(D)J Recombination
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978570 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 55.980, 75.000, 125.910 |
| Unit cell angles | 90.00, 98.13, 90.00 |
Refinement procedure
| Resolution | 44.570 - 2.800 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.24570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jms |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.570 | 2.970 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.060 | 0.673 |
| Number of reflections | 12647 | |
| <I/σ(I)> | 12.67 | 1.75 |
| Completeness [%] | 98.5 | 97.2 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 22-28% PEG 4000, 100-300 mM Lithium Sulfate and 100 mM Tris |
