5CXV
Structure of the human M1 muscarinic acetylcholine receptor bound to antagonist Tiotropium
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Muscarinic acetylcholine receptor M1,Endolysin,Muscarinic acetylcholine receptor M1 | polymer | 515 | 58653.4 | 1 | UniProt (P11229) UniProt (P00720) Pfam (PF00001) Pfam (PF00959) | Homo sapiens (Human) | Lysis protein,Lysozyme,Muramidase |
| 2 | B (C) | FLAG peptide | polymer | 7 | 885.8 | 1 | Enterobacteria phage T4 | ||
| 3 | C (A) | (1R,2R,4S,5S,7S)-7-{[hydroxy(dithiophen-2-yl)acetyl]oxy}-9,9-dimethyl-3-oxa-9-azoniatricyclo[3.3.1.0~2,4~]nonane | non-polymer | 392.5 | 1 | Chemie (0HK) | |||
| 4 | D (A) | CHOLESTEROL HEMISUCCINATE | non-polymer | 486.7 | 1 | Chemie (Y01) | |||
| 5 | E, G, I (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 3 | Chemie (EDO) | |||
| 6 | F (A) | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 1 | Chemie (PGE) | |||
| 7 | H (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 8 | J, K (A, C) | water | water | 18.0 | 10 | Chemie (HOH) |
Sequence modifications
A: 2 - 218 (UniProt: P11229)
A: 1001 - 1160 (UniProt: P00720)
A: 355 - 460 (UniProt: P11229)
| PDB | External Database | Details |
|---|---|---|
| Met -24 | - | initiating methionine |
| Lys -23 | - | expression tag |
| Thr -22 | - | expression tag |
| Ile -21 | - | expression tag |
| Ile -20 | - | expression tag |
| Ala -19 | - | expression tag |
| Leu -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Tyr -16 | - | expression tag |
| Ile -15 | - | expression tag |
| Phe -14 | - | expression tag |
| Cys -13 | - | expression tag |
| Leu -12 | - | expression tag |
| Val -11 | - | expression tag |
| Phe -10 | - | expression tag |
| Ala -9 | - | expression tag |
| Asp -8 | - | expression tag |
| Tyr -7 | - | expression tag |
| Lys -6 | - | expression tag |
| Asp -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Ala 1 | - | expression tag |
| Gln 2 | Asn 2 | engineered mutation |
| Gln 12 | Asn 12 | engineered mutation |
| Gln 110 | Asn 110 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 1011 | Arg 12 | variant |
| Thr 1053 | Cys 54 | engineered mutation |
| Ala 1096 | Cys 97 | engineered mutation |
| Arg 1136 | Ile 137 | variant |
| PDB | External Database | Details |
|---|---|---|
| His 461 | - | expression tag |
| His 462 | - | expression tag |
| His 463 | - | expression tag |
| His 464 | - | expression tag |
| His 465 | - | expression tag |
| His 466 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 59539.2 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 1307.7 | |
| All* | Total formula weight | 60846.9 |
