5CWR
Crystal Structure of human DNA polymerase lambda L431A mutant in complex with a one nucleotide DNA gap and dCTP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | DNA polymerase lambda | polymer | 326 | 36507.6 | 2 | UniProt (Q9UGP5) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791) In PDB | Homo sapiens (Human) | Pol Lambda,DNA polymerase beta-2,Pol beta2,DNA polymerase kappa |
| 2 | T, F | DNA (5'-D(*CP*GP*GP*CP*GP*GP*TP*AP*CP*TP*G)-3') | polymer | 11 | 3390.2 | 2 | synthetic construct | ||
| 3 | P, E | DNA (5'-D(*CP*AP*GP*TP*AP*C)-3') | polymer | 6 | 1793.2 | 2 | synthetic construct | ||
| 4 | D, C | DNA (5'-D(P*GP*CP*CP*G)-3') | polymer | 4 | 1191.8 | 2 | synthetic construct | ||
| 5 | A, B | 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE | non-polymer | 467.2 | 2 | Chemie (DCP) | |||
| 6 | A, B | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
| 7 | water | water | 18.0 | 307 | Chemie (HOH) |
Sequence modifications
A, B: 250 - 575 (UniProt: Q9UGP5)
| PDB | External Database | Details |
|---|---|---|
| Ala 431 | Leu 431 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 85765.7 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1094.6 | |
| All* | Total formula weight | 86860.4 |
