5CJO

Crystal Structure Analysis of Elbow-Engineered-Fab-Bound Human Insulin Degrading Enzyme (IDE) in Complex with Insulin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Insulin-degrading enzyme	polymer	990	114560.6	1	UniProt (P14735) Pfam (PF00675) Pfam (PF05193) Pfam (PF16187) In PDB	Homo sapiens (Human)	Abeta-degrading protease,Insulin protease,Insulinase,Insulysin
2	H	FAB Heavy chain with engineered elbow	polymer	239	25734.8	1		Mus musculus
3	L	FAB light chain	polymer	216	23446.0	1		Mus musculus
4	a	Insulin	polymer	20	2269.6	1	UniProt (P01308) In PDB	Homo sapiens
5	A	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	1	Chemie (EPE)
6	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

A: 42 - 1019 (UniProt: P14735)

PDB	External Database	Details
Met 30	-	expression tag
His 31	-	expression tag
His 32	-	expression tag
His 33	-	expression tag
His 34	-	expression tag
His 35	-	expression tag
His 36	-	expression tag
Ala 37	-	expression tag
Ala 38	-	expression tag
Gly 39	-	expression tag
Ile 40	-	expression tag
Pro 41	-	expression tag
Leu 110	Cys 110	engineered mutation
Gln 111	Glu 111	engineered mutation
Ser 171	Cys 171	engineered mutation
Ala 178	Cys 178	engineered mutation
Val 257	Cys 257	engineered mutation
Leu 414	Cys 414	engineered mutation
Asn 573	Cys 573	engineered mutation
Ser 590	Cys 590	engineered mutation
Ser 789	Cys 789	engineered mutation
Ala 812	Cys 812	engineered mutation
Ala 819	Cys 819	engineered mutation
Ser 904	Cys 904	engineered mutation
Asn 966	Cys 966	engineered mutation
Ala 974	Cys 974	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	166011.0
Non-Polymers*	Number of molecules	2
	Total formula weight	303.7
All*	Total formula weight	166314.7

*Water molecules are not included.