5CC1

S425G Glucocorticoid receptor DNA binding domain - (+)GRE complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, E, F (A, B, W, X)	Glucocorticoid receptor	polymer	114	12729.8	4	UniProt (P04150) Pfam (PF00105)	Homo sapiens (Human)	GR,Nuclear receptor subfamily 3 group C member 1
2	C, H (C, Z)	DNA (5'-D(*CP*CP*AP*GP*AP*AP*CP*AP*GP*AP*GP*TP*GP*TP*TP*CP*TP*G)-3')	polymer	18	5540.6	2		synthetic construct
3	D, G (D, Y)	DNA (5'-D(*TP*CP*AP*GP*AP*AP*CP*AP*CP*TP*CP*TP*GP*TP*TP*CP*TP*G)-3')	polymer	18	5466.6	2		synthetic construct
4	I, J, K, L, M... (A, B, W, X)	ZINC ION	non-polymer		65.4	8	Chemie (ZN)
5	Q, R, S, T (A, B, W, X)	water	water		18.0	20	Chemie (HOH)

Sequence modifications

A, B, W, X: 417 - 506 (UniProt: P04150)

PDB	External Database	Details
Met 393	-	initiating methionine
His 394	-	expression tag
His 395	-	expression tag
His 396	-	expression tag
His 397	-	expression tag
His 398	-	expression tag
His 399	-	expression tag
Ser 400	-	expression tag
Ser 401	-	expression tag
Gly 402	-	expression tag
Val 403	-	expression tag
Asp 404	-	expression tag
Leu 405	-	expression tag
Gly 406	-	expression tag
Thr 407	-	expression tag
Glu 408	-	expression tag
Asn 409	-	expression tag
Leu 410	-	expression tag
Tyr 411	-	expression tag
Phe 412	-	expression tag
Gln 413	-	expression tag
Ser 414	-	expression tag
Asn 415	-	expression tag
Ala 416	-	expression tag
Gly 425	Ser 399	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	72933.4
Non-Polymers*	Number of molecules	8
	Total formula weight	523.3
All*	Total formula weight	73456.7

*Water molecules are not included.