5C55
Crystal structure of the Y138F mutant of C.glutamicum N-acetylneuraminic acid lyase in complex with pyruvate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Dihydrodipicolinate synthase/N-acetylneuraminate lyase | polymer | 312 | 32743.1 | 2 | UniProt (Q8NMD2) Pfam (PF00701) | Corynebacterium glutamicum ATCC 13032 | |
| 2 | C, N (A, B) | PYRUVIC ACID | non-polymer | 88.1 | 2 | Chemie (PYR) | |||
| 3 | D, E, F, G, H... (A, B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 11 | Chemie (EDO) | |||
| 4 | J, K, S (A, B) | ACETATE ION | non-polymer | 59.0 | 3 | Chemie (ACT) | |||
| 5 | O (B) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 6 | T, U (A, B) | water | water | 18.0 | 382 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 312 (UniProt: Q8NMD2)
| PDB | External Database | Details |
|---|---|---|
| Phe 138 | Tyr 138 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 65486.1 | |
| Non-Polymers* | Number of molecules | 17 |
| Total formula weight | 1128.1 | |
| All* | Total formula weight | 66614.2 |
