5C3I

Crystal structure of the quaternary complex of histone H3-H4 heterodimer with chaperone ASF1 and the replicative helicase subunit MCM2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, I, M, Q... (A, E, I, M, Q...)	Histone chaperone ASF1A	polymer	188	21375.6	6	UniProt (Q9Y294) Pfam (PF04729)	Homo sapiens (Human)	Anti-silencing function protein 1 homolog A,hAsf1a,CCG1-interacting factor A,hCIA
2	B, F, J, N, R... (B, F, J, N, R...)	Histone H3.1	polymer	136	15437.2	6	UniProt (P68431) Pfam (PF00125)	Homo sapiens (Human)	Histone H3/a,Histone H3/b,Histone H3/c,Histone H3/d,Histone H3/f,Histone H3/h,Histone H3/i,Histone H3/j,Histone H3/k,Histone H3/l
3	C, G, K, O, S... (C, G, K, O, S...)	Histone H4	polymer	103	11394.4	6	UniProt (P62805) Pfam (PF15511)	Homo sapiens (Human)
4	D, H, L, P, T... (D, H, L, P, T...)	DNA replication licensing factor MCM2,MCM2	polymer	93	9747.7	6	UniProt (P49736) Pfam (PF12619)	Homo sapiens (Human)	Minichromosome maintenance protein 2 homolog,Nuclear protein BM28

Sequence modifications

A, E, I, M, Q, U: 1 - 175 (UniProt: Q9Y294)

PDB	External Database	Details
Met -12	-	expression tag
Gly -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Asn -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag

D, H, L, P, T, X: 63 - 126 (UniProt: P49736)

PDB	External Database	Details
Ser 62	-	expression tag

D, H, L, P, T, X: 127 - 156 (PDB: 5C3I)

Contents of the asymmetric unit

Polymers	Number of chains	24
	Total formula weight	347729.7
All*	Total formula weight	347729.7

*Water molecules are not included.