5C3I

Crystal structure of the quaternary complex of histone H3-H4 heterodimer with chaperone ASF1 and the replicative helicase subunit MCM2

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005634	cellular_component	nucleus
A	0006325	biological_process	chromatin organization
B	0000786	cellular_component	nucleosome
B	0003677	molecular_function	DNA binding
B	0005515	molecular_function	protein binding
B	0005576	cellular_component	extracellular region
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005694	cellular_component	chromosome
B	0006325	biological_process	chromatin organization
B	0006334	biological_process	nucleosome assembly
B	0010467	biological_process	gene expression
B	0016020	cellular_component	membrane
B	0030527	molecular_function	structural constituent of chromatin
B	0032200	biological_process	telomere organization
B	0032991	cellular_component	protein-containing complex
B	0040029	biological_process	epigenetic regulation of gene expression
B	0043229	cellular_component	intracellular organelle
B	0045296	molecular_function	cadherin binding
B	0046982	molecular_function	protein heterodimerization activity
B	0070062	cellular_component	extracellular exosome
C	0000781	cellular_component	chromosome, telomeric region
C	0000786	cellular_component	nucleosome
C	0003677	molecular_function	DNA binding
C	0003723	molecular_function	RNA binding
C	0005515	molecular_function	protein binding
C	0005576	cellular_component	extracellular region
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005694	cellular_component	chromosome
C	0006325	biological_process	chromatin organization
C	0006334	biological_process	nucleosome assembly
C	0016020	cellular_component	membrane
C	0030527	molecular_function	structural constituent of chromatin
C	0032200	biological_process	telomere organization
C	0032991	cellular_component	protein-containing complex
C	0043505	cellular_component	CENP-A containing nucleosome
C	0045653	biological_process	negative regulation of megakaryocyte differentiation
C	0046982	molecular_function	protein heterodimerization activity
C	0061644	biological_process	protein localization to CENP-A containing chromatin
C	0070062	cellular_component	extracellular exosome
D	0003677	molecular_function	DNA binding
D	0005524	molecular_function	ATP binding
D	0005634	cellular_component	nucleus
D	0006270	biological_process	DNA replication initiation
D	0042555	cellular_component	MCM complex
D	1905775	biological_process	negative regulation of DNA helicase activity
E	0005634	cellular_component	nucleus
E	0006325	biological_process	chromatin organization
F	0000786	cellular_component	nucleosome
F	0003677	molecular_function	DNA binding
F	0005515	molecular_function	protein binding
F	0005576	cellular_component	extracellular region
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005694	cellular_component	chromosome
F	0006325	biological_process	chromatin organization
F	0006334	biological_process	nucleosome assembly
F	0010467	biological_process	gene expression
F	0016020	cellular_component	membrane
F	0030527	molecular_function	structural constituent of chromatin
F	0032200	biological_process	telomere organization
F	0032991	cellular_component	protein-containing complex
F	0040029	biological_process	epigenetic regulation of gene expression
F	0043229	cellular_component	intracellular organelle
F	0045296	molecular_function	cadherin binding
F	0046982	molecular_function	protein heterodimerization activity
F	0070062	cellular_component	extracellular exosome
G	0000781	cellular_component	chromosome, telomeric region
G	0000786	cellular_component	nucleosome
G	0003677	molecular_function	DNA binding
G	0003723	molecular_function	RNA binding
G	0005515	molecular_function	protein binding
G	0005576	cellular_component	extracellular region
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005694	cellular_component	chromosome
G	0006325	biological_process	chromatin organization
G	0006334	biological_process	nucleosome assembly
G	0016020	cellular_component	membrane
G	0030527	molecular_function	structural constituent of chromatin
G	0032200	biological_process	telomere organization
G	0032991	cellular_component	protein-containing complex
G	0043505	cellular_component	CENP-A containing nucleosome
G	0045653	biological_process	negative regulation of megakaryocyte differentiation
G	0046982	molecular_function	protein heterodimerization activity
G	0061644	biological_process	protein localization to CENP-A containing chromatin
G	0070062	cellular_component	extracellular exosome
H	0003677	molecular_function	DNA binding
H	0005524	molecular_function	ATP binding
H	0005634	cellular_component	nucleus
H	0006270	biological_process	DNA replication initiation
H	0042555	cellular_component	MCM complex
H	1905775	biological_process	negative regulation of DNA helicase activity
I	0005634	cellular_component	nucleus
I	0006325	biological_process	chromatin organization
J	0000786	cellular_component	nucleosome
J	0003677	molecular_function	DNA binding
J	0005515	molecular_function	protein binding
J	0005576	cellular_component	extracellular region
J	0005634	cellular_component	nucleus
J	0005654	cellular_component	nucleoplasm
J	0005694	cellular_component	chromosome
J	0006325	biological_process	chromatin organization
J	0006334	biological_process	nucleosome assembly
J	0010467	biological_process	gene expression
J	0016020	cellular_component	membrane
J	0030527	molecular_function	structural constituent of chromatin
J	0032200	biological_process	telomere organization
J	0032991	cellular_component	protein-containing complex
J	0040029	biological_process	epigenetic regulation of gene expression
J	0043229	cellular_component	intracellular organelle
J	0045296	molecular_function	cadherin binding
J	0046982	molecular_function	protein heterodimerization activity
J	0070062	cellular_component	extracellular exosome
K	0000781	cellular_component	chromosome, telomeric region
K	0000786	cellular_component	nucleosome
K	0003677	molecular_function	DNA binding
K	0003723	molecular_function	RNA binding
K	0005515	molecular_function	protein binding
K	0005576	cellular_component	extracellular region
K	0005634	cellular_component	nucleus
K	0005654	cellular_component	nucleoplasm
K	0005694	cellular_component	chromosome
K	0006325	biological_process	chromatin organization
K	0006334	biological_process	nucleosome assembly
K	0016020	cellular_component	membrane
K	0030527	molecular_function	structural constituent of chromatin
K	0032200	biological_process	telomere organization
K	0032991	cellular_component	protein-containing complex
K	0043505	cellular_component	CENP-A containing nucleosome
K	0045653	biological_process	negative regulation of megakaryocyte differentiation
K	0046982	molecular_function	protein heterodimerization activity
K	0061644	biological_process	protein localization to CENP-A containing chromatin
K	0070062	cellular_component	extracellular exosome
L	0003677	molecular_function	DNA binding
L	0005524	molecular_function	ATP binding
L	0005634	cellular_component	nucleus
L	0006270	biological_process	DNA replication initiation
L	0042555	cellular_component	MCM complex
L	1905775	biological_process	negative regulation of DNA helicase activity
M	0005634	cellular_component	nucleus
M	0006325	biological_process	chromatin organization
N	0000786	cellular_component	nucleosome
N	0003677	molecular_function	DNA binding
N	0005515	molecular_function	protein binding
N	0005576	cellular_component	extracellular region
N	0005634	cellular_component	nucleus
N	0005654	cellular_component	nucleoplasm
N	0005694	cellular_component	chromosome
N	0006325	biological_process	chromatin organization
N	0006334	biological_process	nucleosome assembly
N	0010467	biological_process	gene expression
N	0016020	cellular_component	membrane
N	0030527	molecular_function	structural constituent of chromatin
N	0032200	biological_process	telomere organization
N	0032991	cellular_component	protein-containing complex
N	0040029	biological_process	epigenetic regulation of gene expression
N	0043229	cellular_component	intracellular organelle
N	0045296	molecular_function	cadherin binding
N	0046982	molecular_function	protein heterodimerization activity
N	0070062	cellular_component	extracellular exosome
O	0000781	cellular_component	chromosome, telomeric region
O	0000786	cellular_component	nucleosome
O	0003677	molecular_function	DNA binding
O	0003723	molecular_function	RNA binding
O	0005515	molecular_function	protein binding
O	0005576	cellular_component	extracellular region
O	0005634	cellular_component	nucleus
O	0005654	cellular_component	nucleoplasm
O	0005694	cellular_component	chromosome
O	0006325	biological_process	chromatin organization
O	0006334	biological_process	nucleosome assembly
O	0016020	cellular_component	membrane
O	0030527	molecular_function	structural constituent of chromatin
O	0032200	biological_process	telomere organization
O	0032991	cellular_component	protein-containing complex
O	0043505	cellular_component	CENP-A containing nucleosome
O	0045653	biological_process	negative regulation of megakaryocyte differentiation
O	0046982	molecular_function	protein heterodimerization activity
O	0061644	biological_process	protein localization to CENP-A containing chromatin
O	0070062	cellular_component	extracellular exosome
P	0003677	molecular_function	DNA binding
P	0005524	molecular_function	ATP binding
P	0005634	cellular_component	nucleus
P	0006270	biological_process	DNA replication initiation
P	0042555	cellular_component	MCM complex
P	1905775	biological_process	negative regulation of DNA helicase activity
Q	0005634	cellular_component	nucleus
Q	0006325	biological_process	chromatin organization
R	0000786	cellular_component	nucleosome
R	0003677	molecular_function	DNA binding
R	0005515	molecular_function	protein binding
R	0005576	cellular_component	extracellular region
R	0005634	cellular_component	nucleus
R	0005654	cellular_component	nucleoplasm
R	0005694	cellular_component	chromosome
R	0006325	biological_process	chromatin organization
R	0006334	biological_process	nucleosome assembly
R	0010467	biological_process	gene expression
R	0016020	cellular_component	membrane
R	0030527	molecular_function	structural constituent of chromatin
R	0032200	biological_process	telomere organization
R	0032991	cellular_component	protein-containing complex
R	0040029	biological_process	epigenetic regulation of gene expression
R	0043229	cellular_component	intracellular organelle
R	0045296	molecular_function	cadherin binding
R	0046982	molecular_function	protein heterodimerization activity
R	0070062	cellular_component	extracellular exosome
S	0000781	cellular_component	chromosome, telomeric region
S	0000786	cellular_component	nucleosome
S	0003677	molecular_function	DNA binding
S	0003723	molecular_function	RNA binding
S	0005515	molecular_function	protein binding
S	0005576	cellular_component	extracellular region
S	0005634	cellular_component	nucleus
S	0005654	cellular_component	nucleoplasm
S	0005694	cellular_component	chromosome
S	0006325	biological_process	chromatin organization
S	0006334	biological_process	nucleosome assembly
S	0016020	cellular_component	membrane
S	0030527	molecular_function	structural constituent of chromatin
S	0032200	biological_process	telomere organization
S	0032991	cellular_component	protein-containing complex
S	0043505	cellular_component	CENP-A containing nucleosome
S	0045653	biological_process	negative regulation of megakaryocyte differentiation
S	0046982	molecular_function	protein heterodimerization activity
S	0061644	biological_process	protein localization to CENP-A containing chromatin
S	0070062	cellular_component	extracellular exosome
T	0003677	molecular_function	DNA binding
T	0005524	molecular_function	ATP binding
T	0005634	cellular_component	nucleus
T	0006270	biological_process	DNA replication initiation
T	0042555	cellular_component	MCM complex
T	1905775	biological_process	negative regulation of DNA helicase activity
U	0005634	cellular_component	nucleus
U	0006325	biological_process	chromatin organization
V	0000786	cellular_component	nucleosome
V	0003677	molecular_function	DNA binding
V	0005515	molecular_function	protein binding
V	0005576	cellular_component	extracellular region
V	0005634	cellular_component	nucleus
V	0005654	cellular_component	nucleoplasm
V	0005694	cellular_component	chromosome
V	0006325	biological_process	chromatin organization
V	0006334	biological_process	nucleosome assembly
V	0010467	biological_process	gene expression
V	0016020	cellular_component	membrane
V	0030527	molecular_function	structural constituent of chromatin
V	0032200	biological_process	telomere organization
V	0032991	cellular_component	protein-containing complex
V	0040029	biological_process	epigenetic regulation of gene expression
V	0043229	cellular_component	intracellular organelle
V	0045296	molecular_function	cadherin binding
V	0046982	molecular_function	protein heterodimerization activity
V	0070062	cellular_component	extracellular exosome
W	0000781	cellular_component	chromosome, telomeric region
W	0000786	cellular_component	nucleosome
W	0003677	molecular_function	DNA binding
W	0003723	molecular_function	RNA binding
W	0005515	molecular_function	protein binding
W	0005576	cellular_component	extracellular region
W	0005634	cellular_component	nucleus
W	0005654	cellular_component	nucleoplasm
W	0005694	cellular_component	chromosome
W	0006325	biological_process	chromatin organization
W	0006334	biological_process	nucleosome assembly
W	0016020	cellular_component	membrane
W	0030527	molecular_function	structural constituent of chromatin
W	0032200	biological_process	telomere organization
W	0032991	cellular_component	protein-containing complex
W	0043505	cellular_component	CENP-A containing nucleosome
W	0045653	biological_process	negative regulation of megakaryocyte differentiation
W	0046982	molecular_function	protein heterodimerization activity
W	0061644	biological_process	protein localization to CENP-A containing chromatin
W	0070062	cellular_component	extracellular exosome
X	0003677	molecular_function	DNA binding
X	0005524	molecular_function	ATP binding
X	0005634	cellular_component	nucleus
X	0006270	biological_process	DNA replication initiation
X	0042555	cellular_component	MCM complex
X	1905775	biological_process	negative regulation of DNA helicase activity

Functional Information from PROSITE/UniProt

site_id	PS00047
Number of Residues	5
Details	HISTONE_H4 Histone H4 signature. GAKRH

Chain	Residue	Details
C	GLY14-HIS18

---

site_id	PS00322
Number of Residues	7
Details	HISTONE_H3_1 Histone H3 signature 1. KAPRKQL

Chain	Residue	Details
B	LYS14-LEU20

---

site_id	PS00959
Number of Residues	9
Details	HISTONE_H3_2 Histone H3 signature 2. PFqRLVREI

Chain	Residue	Details
B	PRO66-ILE74

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	MOD_RES: Phosphoserine; by ATR => ECO:0000269|PubMed:15210935, ECO:0000269|PubMed:16899510, ECO:0007744|PubMed:17525332, ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231

Chain	Residue	Details
D	SER108
H	SER108
L	SER108
P	SER108
T	SER108
X	SER108

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:17967882

Chain	Residue	Details
C	SER1
G	SER1
K	SER1
O	SER1
S	SER1
W	SER1

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	MOD_RES: Symmetric dimethylarginine; by PRMT5 and PRMT7; alternate => ECO:0000250|UniProtKB:P62806

Chain	Residue	Details
C	ARG3
G	ARG3
K	ARG3
O	ARG3
S	ARG3
W	ARG3

---

site_id	SWS_FT_FI4
Number of Residues	6
Details	MOD_RES: N6-lactoyllysine; alternate => ECO:0000269|PubMed:31645732

Chain	Residue	Details
C	LYS5
G	LYS5
K	LYS5
O	LYS5
S	LYS5
W	LYS5

---

site_id	SWS_FT_FI5
Number of Residues	18
Details	MOD_RES: N6-propionyllysine; alternate => ECO:0000269|PubMed:17267393

Chain	Residue	Details
C	LYS8
O	LYS8
O	LYS16
O	LYS44
S	LYS8
S	LYS16
S	LYS44
W	LYS8
W	LYS16
W	LYS44
C	LYS16
C	LYS44
G	LYS8
G	LYS16
G	LYS44
K	LYS8
K	LYS16
K	LYS44

---

site_id	SWS_FT_FI6
Number of Residues	24
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000269|PubMed:22389435

Chain	Residue	Details
C	LYS12
K	LYS31
K	LYS77
K	LYS91
O	LYS12
O	LYS31
O	LYS77
O	LYS91
S	LYS12
S	LYS31
S	LYS77
C	LYS31
S	LYS91
W	LYS12
W	LYS31
W	LYS77
W	LYS91
C	LYS77
C	LYS91
G	LYS12
G	LYS31
G	LYS77
G	LYS91
K	LYS12

---

site_id	SWS_FT_FI7
Number of Residues	6
Details	MOD_RES: N6-methyllysine; alternate => ECO:0000269|PubMed:12086618, ECO:0000269|PubMed:15964846, ECO:0000269|PubMed:17967882, ECO:0000269|PubMed:27338793

Chain	Residue	Details
C	LYS20
G	LYS20
K	LYS20
O	LYS20
S	LYS20
W	LYS20

---

site_id	SWS_FT_FI8
Number of Residues	6
Details	MOD_RES: Phosphoserine; by PAK2 => ECO:0000269|PubMed:21724829, ECO:0007744|PubMed:17081983, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569

Chain	Residue	Details
C	SER47
G	SER47
K	SER47
O	SER47
S	SER47
W	SER47

---

site_id	SWS_FT_FI9
Number of Residues	6
Details	MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:15592455, ECO:0007744|PubMed:20068231

Chain	Residue	Details
C	TYR51
G	TYR51
K	TYR51
O	TYR51
S	TYR51
W	TYR51

---

site_id	SWS_FT_FI10
Number of Residues	6
Details	MOD_RES: N6-glutaryllysine; alternate => ECO:0000269|PubMed:31542297

Chain	Residue	Details
C	LYS59
R	LYS56
V	LYS14
V	LYS56
G	LYS59
K	LYS59
O	LYS59
S	LYS59
W	LYS59
N	LYS14
N	LYS56
R	LYS14

---

site_id	SWS_FT_FI11
Number of Residues	6
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000250|UniProtKB:P62806

Chain	Residue	Details
C	LYS79
G	LYS79
K	LYS79
O	LYS79
S	LYS79
W	LYS79

---

site_id	SWS_FT_FI12
Number of Residues	6
Details	MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:P62806

Chain	Residue	Details
C	THR80
R	LYS64
V	LYS18
V	LYS64
G	THR80
K	THR80
O	THR80
S	THR80
W	THR80
N	LYS18
N	LYS64
R	LYS18

---

site_id	SWS_FT_FI13
Number of Residues	6
Details	MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:24275569

Chain	Residue	Details
C	TYR88
G	TYR88
K	TYR88
O	TYR88
S	TYR88
W	TYR88

---

site_id	SWS_FT_FI14
Number of Residues	6
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate => ECO:0007744|PubMed:25218447

Chain	Residue	Details
C	LYS12
G	LYS12
K	LYS12
O	LYS12
S	LYS12
W	LYS12

---

site_id	SWS_FT_FI15
Number of Residues	12
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000269|PubMed:19818714

Chain	Residue	Details
B	LYS27
S	LYS91
W	LYS91
C	LYS91
J	LYS27
G	LYS91
R	LYS27
K	LYS91
O	LYS91

---

site_id	SWS_FT_FI16
Number of Residues	18
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate => ECO:0007744|PubMed:28112733

Chain	Residue	Details
C	LYS20
O	LYS20
O	LYS59
O	LYS79
S	LYS20
S	LYS59
S	LYS79
W	LYS20
W	LYS59
W	LYS79
C	LYS59
C	LYS79
G	LYS20
G	LYS59
G	LYS79
K	LYS20
K	LYS59
K	LYS79

---

site_id	SWS_FT_FI17
Number of Residues	6
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in UFM1); alternate => ECO:0000269|PubMed:30886146

Chain	Residue	Details
C	LYS31
G	LYS31
K	LYS31
O	LYS31
S	LYS31
W	LYS31

---

site_id	SWS_FT_FI18
Number of Residues	6
Details	MOD_RES: N6-methyllysine => ECO:0000269|PubMed:15983376

Chain	Residue	Details
B	LYS37
F	LYS37
J	LYS37
N	LYS37
R	LYS37
V	LYS37

---

site_id	SWS_FT_FI19
Number of Residues	6
Details	MOD_RES: Phosphotyrosine => ECO:0000269|PubMed:19783980

Chain	Residue	Details
B	TYR41
F	TYR41
J	TYR41
N	TYR41
R	TYR41
V	TYR41

---

site_id	SWS_FT_FI20
Number of Residues	6
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:20850016

Chain	Residue	Details
B	SER57
F	SER57
J	SER57
N	SER57
R	SER57
V	SER57

---

site_id	SWS_FT_FI21
Number of Residues	6
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000269|PubMed:22389435, ECO:0000269|PubMed:29211711

Chain	Residue	Details
B	LYS79
F	LYS79
J	LYS79
N	LYS79
R	LYS79
V	LYS79

---

site_id	SWS_FT_FI22
Number of Residues	6
Details	MOD_RES: Phosphothreonine => ECO:0000269|PubMed:20850016

Chain	Residue	Details
B	THR80
F	THR80
J	THR80
N	THR80
R	THR80
V	THR80

---

site_id	SWS_FT_FI23
Number of Residues	6
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P84243

Chain	Residue	Details
B	SER86
F	SER86
J	SER86
N	SER86
R	SER86
V	SER86

---

site_id	SWS_FT_FI24
Number of Residues	6
Details	MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:Q71DI3

Chain	Residue	Details
B	THR107
F	THR107
J	THR107
N	THR107
R	THR107
V	THR107

---

site_id	SWS_FT_FI25
Number of Residues	6
Details	MOD_RES: N6-glutaryllysine; alternate => ECO:0000269|PubMed:31542297

Chain	Residue	Details
B	LYS115
F	LYS115
J	LYS115
N	LYS115
R	LYS115
V	LYS115

---

site_id	SWS_FT_FI26
Number of Residues	6
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000269|PubMed:22389435, ECO:0000269|PubMed:27436229

Chain	Residue	Details
B	LYS122
F	LYS122
J	LYS122
N	LYS122
R	LYS122
V	LYS122

---

site_id	SWS_FT_FI27
Number of Residues	6
Details	LIPID: N6-decanoyllysine => ECO:0000269|PubMed:35939806

Chain	Residue	Details
B	LYS18
F	LYS18
J	LYS18
N	LYS18
R	LYS18
V	LYS18

---