5C3I
Crystal structure of the quaternary complex of histone H3-H4 heterodimer with chaperone ASF1 and the replicative helicase subunit MCM2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 147.704, 147.704, 261.796 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.990 - 3.500 |
| R-factor | 0.2175 |
| Rwork | 0.216 |
| R-free | 0.25260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2io5 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.915 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 7.530 | 3.500 |
| Rmerge | 0.161 | 0.059 | 0.690 |
| Rmeas | 0.171 | 0.063 | 0.733 |
| Rpim | 0.056 | 0.021 | 0.243 |
| Total number of observations | 389047 | ||
| Number of reflections | 42834 | ||
| <I/σ(I)> | 5.7 | ||
| Completeness [%] | 99.8 | 98.9 | 100 |
| Redundancy | 9.1 | 8.3 | 9.2 |
| CC(1/2) | 0.997 | 0.792 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 20% PEG 3350, 2% Tacsimate,0.1 M Tris-HCl pH 8.0 |
