5C12

Nuclease domain of the large terminase subunit gp2 of bacterial virus Sf6

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Gene 2 protein	polymer	278	30721.6	1	UniProt (Q716H3) In PDB	Enterobacteria phage Sf6 (Shigella flexneri bacteriophage VI)
2	A	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
3		water	water		18.0	330	Chemie (HOH)

Sequence modifications

A: 213 - 470 (UniProt: Q716H3)

PDB	External Database	Details
Met 193	-	expression tag
Gly 194	-	expression tag
Ser 195	-	expression tag
Ser 196	-	expression tag
His 197	-	expression tag
His 198	-	expression tag
His 199	-	expression tag
His 200	-	expression tag
His 201	-	expression tag
His 202	-	expression tag
Ser 203	-	expression tag
Ser 204	-	expression tag
Gly 205	-	expression tag
Leu 206	-	expression tag
Val 207	-	expression tag
Pro 208	-	expression tag
Arg 209	-	expression tag
Gly 210	-	expression tag
Ser 211	-	expression tag
His 212	-	expression tag
Ala 428	Lys 428	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	30721.6
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	30770.2

*Water molecules are not included.