5C12

Nuclease domain of the large terminase subunit gp2 of bacterial virus Sf6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-09-09
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.03316
Spacegroup name	C 1 2 1
Unit cell lengths	132.645, 57.519, 46.598
Unit cell angles	90.00, 98.76, 90.00

Refinement procedure

Resolution	18.971 - 1.517
R-factor	0.1592
Rwork	0.158
R-free	0.18320
RMSD bond length	0.009
RMSD bond angle	1.208
Data scaling software	Aimless (0.1.29)
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.540	29.540	1.520
High resolution limit [Å]	1.490	8.180	1.490
Rmerge	0.095	0.062	2.350
Rpim	0.064	0.045	2.350
Total number of observations	172494	1051	249
Number of reflections	52497
<I/σ(I)>	9.6	22.1	0.1
Completeness [%]	93.1	95.1	8
Redundancy	3.3	3	1.1
CC(1/2)	0.989	0.985

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100mM HEPES, pH7.5, 50mM NaCl, 8% PEG8000