5BWK

6.0 A Crystal structure of a Get3-Get4-Get5 intermediate complex from S.cerevisiae

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, D, M, N...	ATPase GET3	polymer	373	41576.8	8	UniProt (B3LGZ3) Pfam (PF02374) UniProt (by SIFTS) (Q12154) In PDB	Saccharomyces cerevisiae (strain RM11-1a) (Baker's yeast)	Arsenical pump-driving ATPase,Arsenite-stimulated ATPase,Golgi to ER traffic protein 3,Guided entry of tail-anchored proteins 3
2	E, G, I, K, Q...	Golgi to ER traffic protein 4	polymer	319	37246.0	8	UniProt (Q12125) Pfam (PF04190) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Guided entry of tail-anchored proteins 4
3	F, H, J, L, R...	Ubiquitin-like protein MDY2	polymer	56	6305.3	8	UniProt (Q12285) Pfam (PF16843) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Golgi to ER traffic protein 5,Mating-deficient protein 2,Translation machinery-associated protein 24
4	M, O, A, D	ZINC ION	non-polymer		65.4	4	Chemie (ZN)

Sequence modifications

A, B, D, M, N, O, P, C: 2 - 354 (UniProt: B3LGZ3)

PDB	External Database	Details
Met -18	-	initiating methionine
Gly -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Gly -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Ser -1	-	expression tag
Val 0	-	expression tag
Asp 1	-	expression tag

E, G, I, K, Q, S, U, W: 11 - 311 (UniProt: Q12125)

PDB	External Database	Details
Met 9	-	initiating methionine
Gly 10	-	expression tag
Ala 258	Lys 258	conflict
Ala 260	Lys 260	conflict
Gly 312	-	expression tag
Glu 313	-	expression tag
Asn 314	-	expression tag
Leu 315	-	expression tag
Tyr 316	-	expression tag
Phe 317	-	expression tag
Gln 318	-	expression tag
Ser 319	-	expression tag
Leu 320	-	expression tag
Glu 321	-	expression tag
His 322	-	expression tag
His 323	-	expression tag
His 324	-	expression tag
His 325	-	expression tag
His 326	-	expression tag
His 327	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	24
	Total formula weight	681025.3
Non-Polymers*	Number of molecules	4
	Total formula weight	261.6
All*	Total formula weight	681286.9

*Water molecules are not included.