Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5BWK

6.0 A Crystal structure of a Get3-Get4-Get5 intermediate complex from S.cerevisiae

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000750	biological_process	pheromone-dependent signal transduction involved in conjugation with cellular fusion
A	0005085	molecular_function	guanyl-nucleotide exchange factor activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005783	cellular_component	endoplasmic reticulum
A	0005789	cellular_component	endoplasmic reticulum membrane
A	0005794	cellular_component	Golgi apparatus
A	0005829	cellular_component	cytosol
A	0006457	biological_process	protein folding
A	0006620	biological_process	post-translational protein targeting to endoplasmic reticulum membrane
A	0006890	biological_process	retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum
A	0008270	molecular_function	zinc ion binding
A	0009408	biological_process	response to heat
A	0010038	biological_process	response to metal ion
A	0016192	biological_process	vesicle-mediated transport
A	0016787	molecular_function	hydrolase activity
A	0016887	molecular_function	ATP hydrolysis activity
A	0042802	molecular_function	identical protein binding
A	0043529	cellular_component	GET complex
A	0044183	molecular_function	protein folding chaperone
A	0045048	biological_process	protein insertion into ER membrane
A	0046685	biological_process	response to arsenic-containing substance
A	0046872	molecular_function	metal ion binding
A	0051082	molecular_function	unfolded protein binding
A	0071816	biological_process	tail-anchored membrane protein insertion into ER membrane
B	0000166	molecular_function	nucleotide binding
B	0000750	biological_process	pheromone-dependent signal transduction involved in conjugation with cellular fusion
B	0005085	molecular_function	guanyl-nucleotide exchange factor activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005783	cellular_component	endoplasmic reticulum
B	0005789	cellular_component	endoplasmic reticulum membrane
B	0005794	cellular_component	Golgi apparatus
B	0005829	cellular_component	cytosol
B	0006457	biological_process	protein folding
B	0006620	biological_process	post-translational protein targeting to endoplasmic reticulum membrane
B	0006890	biological_process	retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum
B	0008270	molecular_function	zinc ion binding
B	0009408	biological_process	response to heat
B	0010038	biological_process	response to metal ion
B	0016192	biological_process	vesicle-mediated transport
B	0016787	molecular_function	hydrolase activity
B	0016887	molecular_function	ATP hydrolysis activity
B	0042802	molecular_function	identical protein binding
B	0043529	cellular_component	GET complex
B	0044183	molecular_function	protein folding chaperone
B	0045048	biological_process	protein insertion into ER membrane
B	0046685	biological_process	response to arsenic-containing substance
B	0046872	molecular_function	metal ion binding
B	0051082	molecular_function	unfolded protein binding
B	0071816	biological_process	tail-anchored membrane protein insertion into ER membrane
C	0000166	molecular_function	nucleotide binding
C	0000750	biological_process	pheromone-dependent signal transduction involved in conjugation with cellular fusion
C	0005085	molecular_function	guanyl-nucleotide exchange factor activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005783	cellular_component	endoplasmic reticulum
C	0005789	cellular_component	endoplasmic reticulum membrane
C	0005794	cellular_component	Golgi apparatus
C	0005829	cellular_component	cytosol
C	0006457	biological_process	protein folding
C	0006620	biological_process	post-translational protein targeting to endoplasmic reticulum membrane
C	0006890	biological_process	retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum
C	0008270	molecular_function	zinc ion binding
C	0009408	biological_process	response to heat
C	0010038	biological_process	response to metal ion
C	0016192	biological_process	vesicle-mediated transport
C	0016787	molecular_function	hydrolase activity
C	0016887	molecular_function	ATP hydrolysis activity
C	0042802	molecular_function	identical protein binding
C	0043529	cellular_component	GET complex
C	0044183	molecular_function	protein folding chaperone
C	0045048	biological_process	protein insertion into ER membrane
C	0046685	biological_process	response to arsenic-containing substance
C	0046872	molecular_function	metal ion binding
C	0051082	molecular_function	unfolded protein binding
C	0071816	biological_process	tail-anchored membrane protein insertion into ER membrane
D	0000166	molecular_function	nucleotide binding
D	0000750	biological_process	pheromone-dependent signal transduction involved in conjugation with cellular fusion
D	0005085	molecular_function	guanyl-nucleotide exchange factor activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005783	cellular_component	endoplasmic reticulum
D	0005789	cellular_component	endoplasmic reticulum membrane
D	0005794	cellular_component	Golgi apparatus
D	0005829	cellular_component	cytosol
D	0006457	biological_process	protein folding
D	0006620	biological_process	post-translational protein targeting to endoplasmic reticulum membrane
D	0006890	biological_process	retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum
D	0008270	molecular_function	zinc ion binding
D	0009408	biological_process	response to heat
D	0010038	biological_process	response to metal ion
D	0016192	biological_process	vesicle-mediated transport
D	0016787	molecular_function	hydrolase activity
D	0016887	molecular_function	ATP hydrolysis activity
D	0042802	molecular_function	identical protein binding
D	0043529	cellular_component	GET complex
D	0044183	molecular_function	protein folding chaperone
D	0045048	biological_process	protein insertion into ER membrane
D	0046685	biological_process	response to arsenic-containing substance
D	0046872	molecular_function	metal ion binding
D	0051082	molecular_function	unfolded protein binding
D	0071816	biological_process	tail-anchored membrane protein insertion into ER membrane
E	0003674	molecular_function	molecular_function
E	0005515	molecular_function	protein binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006620	biological_process	post-translational protein targeting to endoplasmic reticulum membrane
E	0016192	biological_process	vesicle-mediated transport
E	0045048	biological_process	protein insertion into ER membrane
E	0072380	cellular_component	TRC complex
G	0003674	molecular_function	molecular_function
G	0005515	molecular_function	protein binding
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006620	biological_process	post-translational protein targeting to endoplasmic reticulum membrane
G	0016192	biological_process	vesicle-mediated transport
G	0045048	biological_process	protein insertion into ER membrane
G	0072380	cellular_component	TRC complex
I	0003674	molecular_function	molecular_function
I	0005515	molecular_function	protein binding
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0006620	biological_process	post-translational protein targeting to endoplasmic reticulum membrane
I	0016192	biological_process	vesicle-mediated transport
I	0045048	biological_process	protein insertion into ER membrane
I	0072380	cellular_component	TRC complex
K	0003674	molecular_function	molecular_function
K	0005515	molecular_function	protein binding
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0006620	biological_process	post-translational protein targeting to endoplasmic reticulum membrane
K	0016192	biological_process	vesicle-mediated transport
K	0045048	biological_process	protein insertion into ER membrane
K	0072380	cellular_component	TRC complex
M	0000166	molecular_function	nucleotide binding
M	0000750	biological_process	pheromone-dependent signal transduction involved in conjugation with cellular fusion
M	0005085	molecular_function	guanyl-nucleotide exchange factor activity
M	0005515	molecular_function	protein binding
M	0005524	molecular_function	ATP binding
M	0005737	cellular_component	cytoplasm
M	0005783	cellular_component	endoplasmic reticulum
M	0005789	cellular_component	endoplasmic reticulum membrane
M	0005794	cellular_component	Golgi apparatus
M	0005829	cellular_component	cytosol
M	0006457	biological_process	protein folding
M	0006620	biological_process	post-translational protein targeting to endoplasmic reticulum membrane
M	0006890	biological_process	retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum
M	0008270	molecular_function	zinc ion binding
M	0009408	biological_process	response to heat
M	0010038	biological_process	response to metal ion
M	0016192	biological_process	vesicle-mediated transport
M	0016787	molecular_function	hydrolase activity
M	0016887	molecular_function	ATP hydrolysis activity
M	0042802	molecular_function	identical protein binding
M	0043529	cellular_component	GET complex
M	0044183	molecular_function	protein folding chaperone
M	0045048	biological_process	protein insertion into ER membrane
M	0046685	biological_process	response to arsenic-containing substance
M	0046872	molecular_function	metal ion binding
M	0051082	molecular_function	unfolded protein binding
M	0071816	biological_process	tail-anchored membrane protein insertion into ER membrane
N	0000166	molecular_function	nucleotide binding
N	0000750	biological_process	pheromone-dependent signal transduction involved in conjugation with cellular fusion
N	0005085	molecular_function	guanyl-nucleotide exchange factor activity
N	0005515	molecular_function	protein binding
N	0005524	molecular_function	ATP binding
N	0005737	cellular_component	cytoplasm
N	0005783	cellular_component	endoplasmic reticulum
N	0005789	cellular_component	endoplasmic reticulum membrane
N	0005794	cellular_component	Golgi apparatus
N	0005829	cellular_component	cytosol
N	0006457	biological_process	protein folding
N	0006620	biological_process	post-translational protein targeting to endoplasmic reticulum membrane
N	0006890	biological_process	retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum
N	0008270	molecular_function	zinc ion binding
N	0009408	biological_process	response to heat
N	0010038	biological_process	response to metal ion
N	0016192	biological_process	vesicle-mediated transport
N	0016787	molecular_function	hydrolase activity
N	0016887	molecular_function	ATP hydrolysis activity
N	0042802	molecular_function	identical protein binding
N	0043529	cellular_component	GET complex
N	0044183	molecular_function	protein folding chaperone
N	0045048	biological_process	protein insertion into ER membrane
N	0046685	biological_process	response to arsenic-containing substance
N	0046872	molecular_function	metal ion binding
N	0051082	molecular_function	unfolded protein binding
N	0071816	biological_process	tail-anchored membrane protein insertion into ER membrane
O	0000166	molecular_function	nucleotide binding
O	0000750	biological_process	pheromone-dependent signal transduction involved in conjugation with cellular fusion
O	0005085	molecular_function	guanyl-nucleotide exchange factor activity
O	0005515	molecular_function	protein binding
O	0005524	molecular_function	ATP binding
O	0005737	cellular_component	cytoplasm
O	0005783	cellular_component	endoplasmic reticulum
O	0005789	cellular_component	endoplasmic reticulum membrane
O	0005794	cellular_component	Golgi apparatus
O	0005829	cellular_component	cytosol
O	0006457	biological_process	protein folding
O	0006620	biological_process	post-translational protein targeting to endoplasmic reticulum membrane
O	0006890	biological_process	retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum
O	0008270	molecular_function	zinc ion binding
O	0009408	biological_process	response to heat
O	0010038	biological_process	response to metal ion
O	0016192	biological_process	vesicle-mediated transport
O	0016787	molecular_function	hydrolase activity
O	0016887	molecular_function	ATP hydrolysis activity
O	0042802	molecular_function	identical protein binding
O	0043529	cellular_component	GET complex
O	0044183	molecular_function	protein folding chaperone
O	0045048	biological_process	protein insertion into ER membrane
O	0046685	biological_process	response to arsenic-containing substance
O	0046872	molecular_function	metal ion binding
O	0051082	molecular_function	unfolded protein binding
O	0071816	biological_process	tail-anchored membrane protein insertion into ER membrane
P	0000166	molecular_function	nucleotide binding
P	0000750	biological_process	pheromone-dependent signal transduction involved in conjugation with cellular fusion
P	0005085	molecular_function	guanyl-nucleotide exchange factor activity
P	0005515	molecular_function	protein binding
P	0005524	molecular_function	ATP binding
P	0005737	cellular_component	cytoplasm
P	0005783	cellular_component	endoplasmic reticulum
P	0005789	cellular_component	endoplasmic reticulum membrane
P	0005794	cellular_component	Golgi apparatus
P	0005829	cellular_component	cytosol
P	0006457	biological_process	protein folding
P	0006620	biological_process	post-translational protein targeting to endoplasmic reticulum membrane
P	0006890	biological_process	retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum
P	0008270	molecular_function	zinc ion binding
P	0009408	biological_process	response to heat
P	0010038	biological_process	response to metal ion
P	0016192	biological_process	vesicle-mediated transport
P	0016787	molecular_function	hydrolase activity
P	0016887	molecular_function	ATP hydrolysis activity
P	0042802	molecular_function	identical protein binding
P	0043529	cellular_component	GET complex
P	0044183	molecular_function	protein folding chaperone
P	0045048	biological_process	protein insertion into ER membrane
P	0046685	biological_process	response to arsenic-containing substance
P	0046872	molecular_function	metal ion binding
P	0051082	molecular_function	unfolded protein binding
P	0071816	biological_process	tail-anchored membrane protein insertion into ER membrane
Q	0003674	molecular_function	molecular_function
Q	0005515	molecular_function	protein binding
Q	0005737	cellular_component	cytoplasm
Q	0005829	cellular_component	cytosol
Q	0006620	biological_process	post-translational protein targeting to endoplasmic reticulum membrane
Q	0016192	biological_process	vesicle-mediated transport
Q	0045048	biological_process	protein insertion into ER membrane
Q	0072380	cellular_component	TRC complex
S	0003674	molecular_function	molecular_function
S	0005515	molecular_function	protein binding
S	0005737	cellular_component	cytoplasm
S	0005829	cellular_component	cytosol
S	0006620	biological_process	post-translational protein targeting to endoplasmic reticulum membrane
S	0016192	biological_process	vesicle-mediated transport
S	0045048	biological_process	protein insertion into ER membrane
S	0072380	cellular_component	TRC complex
U	0003674	molecular_function	molecular_function
U	0005515	molecular_function	protein binding
U	0005737	cellular_component	cytoplasm
U	0005829	cellular_component	cytosol
U	0006620	biological_process	post-translational protein targeting to endoplasmic reticulum membrane
U	0016192	biological_process	vesicle-mediated transport
U	0045048	biological_process	protein insertion into ER membrane
U	0072380	cellular_component	TRC complex
W	0003674	molecular_function	molecular_function
W	0005515	molecular_function	protein binding
W	0005737	cellular_component	cytoplasm
W	0005829	cellular_component	cytosol
W	0006620	biological_process	post-translational protein targeting to endoplasmic reticulum membrane
W	0016192	biological_process	vesicle-mediated transport
W	0045048	biological_process	protein insertion into ER membrane
W	0072380	cellular_component	TRC complex

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	binding site for residue ZN A 500

Chain	Residue
A	CYS285
B	CYS285
B	CYS288

site_id	AC2
Number of Residues	5
Details	binding site for residue ZN D 501

Chain	Residue
C	CYS285
C	ARG287
C	CYS288
D	CYS285
D	CYS288

site_id	AC3
Number of Residues	5
Details	binding site for residue ZN M 401

Chain	Residue
M	CYS288
N	CYS285
N	ARG287
N	CYS288
M	CYS285

site_id	AC4
Number of Residues	5
Details	binding site for residue ZN O 401

Chain	Residue
O	CYS285
O	CYS288
P	CYS285
P	ARG287
P	CYS288

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE:

Chain	Residue	Details
A	ASP57
B	ASP57
D	ASP57
M	ASP57
N	ASP57
O	ASP57
P	ASP57
C	ASP57

site_id	SWS_FT_FI2
Number of Residues	32
Details	BINDING:

Chain	Residue	Details
A	LYS26
M	LYS26
M	GLU245
M	ASN272
N	LYS26
N	GLU245
N	ASN272
O	LYS26
O	GLU245
O	ASN272
P	LYS26
A	GLU245
P	GLU245
P	ASN272
C	LYS26
C	GLU245
C	ASN272
A	PRO315
B	PRO315
D	PRO315
M	PRO315
N	PRO315
A	ASN272
O	PRO315
P	PRO315
C	PRO315
B	LYS26
B	GLU245
B	ASN272
D	LYS26
D	GLU245
D	ASN272

site_id	SWS_FT_FI3
Number of Residues	16
Details	BINDING: BINDING => ECO:0000255\|HAMAP-Rule:MF_03112, ECO:0000269\|PubMed:19675567

Chain	Residue	Details
A	CYS285
N	CYS288
O	CYS285
O	CYS288
P	CYS285
P	CYS288
C	CYS285
C	CYS288
A	CYS288
B	CYS285
B	CYS288
D	CYS285
D	CYS288
M	CYS285
M	CYS288
N	CYS285

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)