5BWK
6.0 A Crystal structure of a Get3-Get4-Get5 intermediate complex from S.cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-06-20 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | .9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 152.430, 127.330, 210.260 |
| Unit cell angles | 90.00, 110.23, 90.00 |
Refinement procedure
| Resolution | 30.000 - 6.000 |
| R-factor | 0.274 |
| Rwork | 0.274 |
| R-free | 0.30250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3A37 and 3LKU |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.737 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 6.600 |
| High resolution limit [Å] | 6.000 | 6.000 |
| Rmerge | 0.060 | 0.780 |
| Number of reflections | 18388 | |
| <I/σ(I)> | 8.9 | 1.4 |
| Completeness [%] | 96.3 | 98.7 |
| Redundancy | 3.2 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 12% PEG 3350 and 0.1 M Na malonate (pH 5.0) |
