5BV7
Crystal structure of human LCAT (L4F, N5D) in complex with Fab of an agonistic antibody
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Phosphatidylcholine-sterol acyltransferase | polymer | 422 | 47958.5 | 1 | UniProt (P04180) Pfam (PF02450) | Homo sapiens (Human) | Lecithin-cholesterol acyltransferase,Phospholipid-cholesterol acyltransferase |
| 2 | B (L) | 27C3 light chain | polymer | 214 | 22801.1 | 1 | Homo sapiens (Human) | ||
| 3 | C (H) | 27C3 heavy chain | polymer | 233 | 24696.6 | 1 | Homo sapiens (Human) | ||
| 4 | D (B) | Fab1 light chain | polymer | 213 | 22744.1 | 1 | Homo sapiens (Human) | ||
| 5 | E (C) | Fab1 heavy chain | polymer | 238 | 25644.6 | 1 | Homo sapiens (Human) | ||
| 6 | F (D) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1235.1 | 1 | In PDB GlyTouCan (G09609EN) | |||
| 7 | G, H (A) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 2 | Chemie (NAG) | |||
| 8 | I, J, K, L, M (A, L, H, B, C) | water | water | 18.0 | 388 | Chemie (HOH) |
Sequence modifications
A: 1 - 416 (UniProt: P04180)
| PDB | External Database | Details |
|---|---|---|
| Phe 4 | Leu 28 | engineered mutation |
| Asp 5 | Asn 29 | engineered mutation |
| Glu 417 | - | expression tag |
| Asn 418 | - | expression tag |
| Leu 419 | - | expression tag |
| Tyr 420 | - | expression tag |
| Phe 421 | - | expression tag |
| Gln 422 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 143844.8 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 1235.1 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 442.4 | |
| All* | Total formula weight | 145522.4 |
