5BUU

Crystal structure of the GluA2 ligand-binding domain (L483Y-N754S) in complex with glutamate and BPAM-321 at 2.07 A resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Glutamate receptor 2,Glutamate receptor 2	polymer	263	29244.7	2	UniProt (P19491) Pfam (PF10613) Pfam (PF00060) In PDB	Rattus norvegicus (Norway Rat)	GluR-2,AMPA-selective glutamate receptor 2,GluR-B,GluR-K2,Glutamate receptor ionotropic,AMPA 2,GluA2,GluR-2,AMPA-selective glutamate receptor 2,GluR-B,GluR-K2,Glutamate receptor ionotropic,AMPA 2,GluA2
2	A, B	(3R)-7-chloro-2,3,4-trimethyl-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide	non-polymer		260.7	2	Chemie (4V6)
3	A, B	GLUTAMIC ACID	non-polymer		147.1	2	Chemie (GLU)
4	A, B	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)
5	B	1,2-ETHANEDIOL	non-polymer		62.1	5	Chemie (EDO)
6		water	water		18.0	275	Chemie (HOH)

Sequence modifications

A, B: 392 - 506 (UniProt: P19491)

PDB	External Database	Details
Gly 390	-	expression tag
Ala 391	-	expression tag
Tyr 483	Leu 504	engineered mutation

A, B: 632 - 775 (UniProt: P19491)

PDB	External Database	Details
Gly 507	-	linker
Thr 508	-	linker
Ser 754	Asn 775	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	58489.4
Non-Polymers*	Number of molecules	14
	Total formula weight	1606.4
All*	Total formula weight	60095.7

*Water molecules are not included.