5BQC

Crystal structure of Norrin in complex with the cysteine-rich domain of Frizzled 4 and sucrose octasulfate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B	Frizzled-4	polymer	149	16705.2	1	UniProt (Q9ULV1) Pfam (PF01392) In PDB	Homo sapiens (Human)	hFz4,FzE4
2	A	Norrin	polymer	122	13614.7	1	UniProt (Q00604) Pfam (PF00007) In PDB	Homo sapiens (Human)	Norrie disease protein,X-linked exudative vitreoretinopathy 2 protein
3	C	1,3,4,6-tetra-O-sulfo-beta-D-fructofuranose-(2-1)-2,3,4,6-tetra-O-sulfonato-alpha-D-glucopyranose	branched		982.8	1	In PDB BIRD (PRD_900013)		sucrose octasulfate
4	B	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)

Sequence modifications

B: 42 - 179 (UniProt: Q9ULV1)

PDB	External Database	Details
Asp 39	-	expression tag
Thr 40	-	expression tag
Gly 41	-	expression tag
Gly 180	-	expression tag
Thr 181	-	expression tag
Leu 182	-	expression tag
Glu 183	-	expression tag
Val 184	-	expression tag
Leu 185	-	expression tag
Phe 186	-	expression tag
Gln 187	-	expression tag

A: 25 - 133 (UniProt: Q00604)

PDB	External Database	Details
Gly 22	-	expression tag
Pro 23	-	expression tag
Gly 24	-	expression tag
Gly 134	-	expression tag
Thr 135	-	expression tag
Glu 136	-	expression tag
Thr 137	-	expression tag
Ser 138	-	expression tag
Gln 139	-	expression tag
Val 140	-	expression tag
Ala 141	-	expression tag
Pro 142	-	expression tag
Ala 143	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	30319.9
Branched	Number of molecules	1
	Total formula weight	982.8
Non-Polymers*	Number of molecules	2
	Total formula weight	442.4
All*	Total formula weight	31745.2

*Water molecules are not included.