5BQC
Crystal structure of Norrin in complex with the cysteine-rich domain of Frizzled 4 and sucrose octasulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 119.136, 119.136, 119.151 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.340 - 3.000 |
| R-factor | 0.2175 |
| Rwork | 0.215 |
| R-free | 0.26760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bpb 5bpu |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.181 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.2.17) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.340 | 47.340 | 3.180 |
| High resolution limit [Å] | 3.000 | 9.000 | 3.000 |
| Rmerge | 0.130 | 0.049 | 2.460 |
| Rpim | 0.031 | 0.013 | 0.548 |
| Total number of observations | 205532 | 7834 | 34026 |
| Number of reflections | 10503 | ||
| <I/σ(I)> | 14.6 | 50.2 | 1.6 |
| Completeness [%] | 100.0 | 99.1 | 100 |
| Redundancy | 19.6 | 16.8 | 20.6 |
| CC(1/2) | 0.999 | 0.999 | 0.149 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1 M Tris, pH 8.0, 0.15 M NaCl, 8% PEG8000 |
