5B2G

Crystal structure of human claudin-4 in complex with C-terminal fragment of Clostridium perfringens enterotoxin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G	Endolysin,Claudin-4	polymer	364	39410.8	4	UniProt (P00720) UniProt (O14493) Pfam (PF00959) Pfam (PF00822) In PDB	Enterobacteria phage T4	Lysis protein,Lysozyme,Muramidase,Clostridium perfringens enterotoxin receptor,CPE-receptor,Williams-Beuren syndrome chromosomal region 8 protein
2	B, D, F, H	Heat-labile enterotoxin B chain	polymer	142	15879.5	4	UniProt (P01558) In PDB	Clostridium perfringens

Sequence modifications

A, C, E, G: 1002 - 1162 (UniProt: P00720)

PDB	External Database	Details
Gly 995	-	expression tag
Ser 996	-	expression tag
Ser 997	-	expression tag
Gly 998	-	expression tag
Ser 999	-	expression tag
Ser 1000	-	expression tag
Gly 1001	-	expression tag
Gly 1012	Arg 12	engineered mutation
Thr 1054	Cys 54	engineered mutation
Ala 1097	Cys 97	engineered mutation
Arg 1137	Ile 137	engineered mutation
Gly 1163	-	linker

A, C, E, G: 1164 - 1346 (UniProt: O14493)

PDB	External Database	Details
Ser 1347	-	expression tag
Gly 1348	-	expression tag
Pro 1349	-	expression tag
Ser 1350	-	expression tag
Ser 1351	-	expression tag
Gly 1352	-	expression tag
Glu 1353	-	expression tag
Asn 1354	-	expression tag
Leu 1355	-	expression tag
Tyr 1356	-	expression tag
Phe 1357	-	expression tag
Gln 1358	-	expression tag

B, D, F, H: 187 - 319 (UniProt: P01558)

PDB	External Database	Details
Gly 178	-	expression tag
Ser 179	-	expression tag
Ser 180	-	expression tag
Gly 181	-	expression tag
Ser 182	-	expression tag
Ser 183	-	expression tag
Gly 184	-	expression tag
Arg 185	-	expression tag
Trp 186	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	221161.2
All*	Total formula weight	221161.2

*Water molecules are not included.