5B2G
Crystal structure of human claudin-4 in complex with C-terminal fragment of Clostridium perfringens enterotoxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-13 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 43 |
| Unit cell lengths | 105.920, 105.920, 244.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.589 - 3.500 |
| R-factor | 0.2892 |
| Rwork | 0.288 |
| R-free | 0.30870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3am2 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.172 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.350 |
| Rmerge | 0.161 |
| Number of reflections | 76023 |
| <I/σ(I)> | 6.63 |
| Completeness [%] | 99.6 |
| Redundancy | 4.56 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | 75mM MES-NaOH, 20% PEG3350, 7-10% 1,6-hexanediol, 0.002% NaN3, 0.0005% 2,6-di-t-butyl-p-cresol, 150mM NaCl |
