5AXW
Crystal structure of Staphylococcus aureus Cas9 in complex with sgRNA and target DNA (TTGGGT PAM)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | CRISPR-associated endonuclease Cas9 | polymer | 1056 | 124365.9 | 1 | UniProt (J7RUA5) Pfam (PF13395) Pfam (PF22702) Pfam (PF18061) Pfam (PF18070) Pfam (PF21574) In PDB | Staphylococcus aureus subsp. aureus | |
| 2 | B | RNA (73-MER) | polymer | 73 | 23526.0 | 1 | Staphylococcus aureus | ||
| 3 | C | DNA (28-MER) | polymer | 28 | 8439.5 | 1 | Staphylococcus aureus | ||
| 4 | D | DNA (5'-D(*TP*TP*GP*GP*GP*TP*AP*G)-3') | polymer | 8 | 2497.7 | 1 | Staphylococcus aureus | ||
| 5 | A, B | SODIUM ION | non-polymer | 23.0 | 4 | Chemie (NA) | |||
| 6 | B | PHOSPHATE ION | non-polymer | 95.0 | 1 | Chemie (PO4) | |||
| 7 | B, D | 1,2-ETHANEDIOL | non-polymer | 62.1 | 2 | Chemie (EDO) | |||
| 8 | water | water | 18.0 | 76 | Chemie (HOH) |
Sequence modifications
A: 1 - 1053 (UniProt: J7RUA5)
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Ala 580 | Asn 580 | engineered mutation |
| Ala 946 | Cys 946 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 158829.0 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 311.1 | |
| All* | Total formula weight | 159140.1 |
