5AWH

Rhodobacter sphaeroides Argonaute in complex with guide RNA/target DNA heteroduplex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Uncharacterized protein	polymer	778	87143.5	2	UniProt (A4WYU7) Pfam (PF02171)	Rhodobacter sphaeroides	Argonaute
2	C, E (C, E)	RNA (5'-D(P*UP*UP*AP*CP*AP*AP*CP*CP*UP*AP*CP*UP*AP*CP*CP*UP*CP*G)-3')	polymer	18	5613.4	2		synthetic construct
3	D, F (D, F)	DNA (5'-D(*CP*GP*AP*GP*GP*TP*AP*GP*TP*AP*GP*GP*TP*TP*GP*TP*AP*A)-3')	polymer	18	5635.7	2		synthetic construct
4	G, H (C, E)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	I, J, K, L, M... (A, B, C, D, E...)	water	water		18.0	636	Chemie (HOH)

Sequence modifications

A, B: 21 - 777 (UniProt: A4WYU7)

PDB	External Database	Details
Met 0	-	expression tag
Gly 1	-	expression tag
Ser 2	-	expression tag
Ser 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
Ser 10	-	expression tag
Ser 11	-	expression tag
Gly 12	-	expression tag
Leu 13	-	expression tag
Val 14	-	expression tag
Pro 15	-	expression tag
Ala 16	-	expression tag
Gly 17	-	expression tag
Ser 18	-	expression tag
His 19	-	expression tag
Met 20	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	196785.0
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	196833.6

*Water molecules are not included.