5AWH
Rhodobacter sphaeroides Argonaute in complex with guide RNA/target DNA heteroduplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-09 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.186, 118.303, 118.480 |
| Unit cell angles | 90.00, 95.83, 90.00 |
Refinement procedure
| Resolution | 35.630 - 2.000 |
| R-factor | 0.1866 |
| Rwork | 0.184 |
| R-free | 0.23250 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.758 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.063 | 0.047 | 0.400 |
| Rmeas | 0.074 | ||
| Rpim | 0.039 | ||
| Total number of observations | 466156 | ||
| Number of reflections | 123866 | ||
| <I/σ(I)> | 15.3 | ||
| Completeness [%] | 97.7 | 74.6 | 100 |
| Redundancy | 3.8 | 3.4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.05 M sodium cacodylate trihydrlate (pH 7.0), 42% (v/v) 2-methyl-2,4-pentanediol |
