5AHI

Crystal structure of salmonalla enterica HisA mutant D7N with ProFAR

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO) METHYLIDENE AMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE	polymer	253	27130.9	1	UniProt (P10372) Pfam (PF00977) In PDB	SALMONELLA ENTERICA	PHOSPHORIBOSYLFORMIMINO-5-AMINOIMIDAZOLE CARBOXAMIDE RIBOTIDE ISOMERASE, HISA
2	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
3	A	[(2R,3S,4R,5R)-5-[4-aminocarbonyl-5-[(E)-[[(2R,3R,4S,5R)-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]amino]methylideneamino]imidazol-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl dihydrogen phosphate	non-polymer		577.3	1	Chemie (GUO)
4	A	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
5	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6		water	water		18.0	106	Chemie (HOH)

Sequence modifications

A: 1 - 245 (UniProt: P10372)

PDB	External Database	Details
Asn 7	Asp 7	engineered mutation
Lys 246	-	expression tag
Gly 247	-	expression tag
His 248	-	expression tag
His 249	-	expression tag
His 250	-	expression tag
His 251	-	expression tag
His 252	-	expression tag
His 253	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	27130.9
Non-Polymers*	Number of molecules	4
	Total formula weight	799.8
All*	Total formula weight	27930.8

*Water molecules are not included.