5AHI
Crystal structure of salmonalla enterica HisA mutant D7N with ProFAR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-27 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 85.593, 85.593, 121.310 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.942 - 1.999 |
| R-factor | 0.1965 |
| Rwork | 0.194 |
| R-free | 0.24710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | THE WILD TYPE S. ENTERICA HISA |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.137 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.940 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.140 | 0.790 |
| Number of reflections | 17983 | |
| <I/σ(I)> | 13.8 | 2.94 |
| Completeness [%] | 97.5 | 79.1 |
| Redundancy | 8.5 | 8.01 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.2M LITHIUM SULFATE, 30% W/V PEG 8K, 0.1M SODIUM ACETATE PH4.5, PH 7 |
