5AB3

S.enterica HisA mutant D7N, D10G, dup13-15, Q24L, G102A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase	polymer	256	27427.4	3	UniProt (V7IJE3) Pfam (PF00977) UniProt (by SIFTS) (P10372)	Salmonella enterica subsp. enterica serovar Cubana str. 76814	Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase
2	D, F, I (A, B, C)	[(2R,3S,4R,5R)-5-[4-AMINOCARBONYL-5-[[(Z)-[(3R,4R)-3,4-DIHYDROXY-2-OXO-5-PHOSPHONOOXY-PENTYL]IMINOMETHYL]AMINO]IMIDAZOL-1-YL]-3,4-DIHYDROXY-OXOLAN-2-YL]METHYL DIHYDROGEN PHOSPHATE	non-polymer		577.3	3	Chemie (2ER)
3	E, G, H, J (A, B, C)	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
4	K, L, M (A, B, C)	water	water		18.0	532	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 245 (UniProt: V7IJE3)

PDB	External Database	Details
Asn 7	Asp 7	engineered mutation
Gly 10	Asp 10	engineered mutation
Val 13	-	insertion
Val 14	-	insertion
Arg 15	-	insertion
Leu 24	Gln 24	engineered mutation
Ala 102	Gly 102	engineered mutation
Lys 246	-	expression tag
Gly 247	-	expression tag
His 248	-	expression tag
His 249	-	expression tag
His 250	-	expression tag
His 251	-	expression tag
His 252	-	expression tag
His 253	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	82282.3
Non-Polymers*	Number of molecules	7
	Total formula weight	1824.0
All*	Total formula weight	84106.2

*Water molecules are not included.