4ZT9

Nuclease-inactive Streptococcus pyogenes Cas9 (D10A/H840A, dCas9) in complex with single-guide RNA at 3.1 Angstrom resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	CRISPR-associated endonuclease Cas9	polymer	1369	158661.8	2	UniProt (A0A0C6FZC2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) UniProt (by SIFTS) (Q99ZW2)	Streptococcus pyogenes	Cas9, CRISPR-Cas9
2	B, D (B, D)	single-guide RNA	polymer	85	27501.4	2		Streptococcus pyogenes

Sequence modifications

A, C: 1 - 1368 (UniProt: A0A0C6FZC2)

PDB	External Database	Details
Ser 0	-	expression tag
Ala 10	Asp 10	engineered mutation
Ala 840	His 840	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	372326.4
All*	Total formula weight	372326.4

*Water molecules are not included.