4ZT9

Nuclease-inactive Streptococcus pyogenes Cas9 (D10A/H840A, dCas9) in complex with single-guide RNA at 3.1 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	102.505, 143.046, 296.145
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.357 - 3.100
R-factor	0.245
Rwork	0.243
R-free	0.28410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zt0
RMSD bond length	0.008
RMSD bond angle	0.911
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	102.500	3.270
High resolution limit [Å]	3.100	3.100
Rmerge	0.068	0.499
Number of reflections	76812
<I/σ(I)>	14.5
Completeness [%]	96.2	89.8
Redundancy	40

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	12% w/v PEG8000, 100 mM sodium cacodylate, pH 6.0, 250 mM sodium chloride