4ZT9

Nuclease-inactive Streptococcus pyogenes Cas9 (D10A/H840A, dCas9) in complex with single-guide RNA at 3.1 Angstrom resolution

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003723	molecular_function	RNA binding
A	0004519	molecular_function	endonuclease activity
A	0004520	molecular_function	DNA endonuclease activity
A	0004527	molecular_function	exonuclease activity
A	0008408	molecular_function	3'-5' exonuclease activity
A	0043571	biological_process	maintenance of CRISPR repeat elements
A	0046872	molecular_function	metal ion binding
A	0051607	biological_process	defense response to virus
C	0003677	molecular_function	DNA binding
C	0003723	molecular_function	RNA binding
C	0004519	molecular_function	endonuclease activity
C	0004520	molecular_function	DNA endonuclease activity
C	0004527	molecular_function	exonuclease activity
C	0008408	molecular_function	3'-5' exonuclease activity
C	0043571	biological_process	maintenance of CRISPR repeat elements
C	0046872	molecular_function	metal ion binding
C	0051607	biological_process	defense response to virus

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: For RuvC-like nuclease domain => ECO:0000305|PubMed:24529477

Chain	Residue	Details
A	ALA10
C	ALA10

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site_id	SWS_FT_FI2
Number of Residues	2
Details	ACT_SITE: Proton acceptor for HNH nuclease domain => ECO:0000305|PubMed:24529477

Chain	Residue	Details
A	ALA840
C	ALA840

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site_id	SWS_FT_FI3
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269|PubMed:24505130

Chain	Residue	Details
A	ALA10
C	HIS983
C	HIS1297
C	ASP1328
A	GLU762
A	GLU766
A	HIS983
A	HIS1297
A	ASP1328
C	ALA10
C	GLU762
C	GLU766

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