4ZM9

Crystal structure of circularly permuted human asparaginase-like protein 1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Isoaspartyl peptidase/L-asparaginase	polymer	327	33767.2	4	UniProt (Q7L266) Pfam (PF01112) In PDB	Homo sapiens (Human)	Asparaginase-like protein 1,Beta-aspartyl-peptidase,Isoaspartyl dipeptidase,L-asparagine amidohydrolase
2	A, B, C, D	SODIUM ION	non-polymer		23.0	7	Chemie (NA)
3	D, A, B, C	GLYCINE	non-polymer		75.1	4	Chemie (GLY)
4	D, A, B, C	BETA-MERCAPTOETHANOL	non-polymer		78.1	10	Chemie (BME)
5	A, C	2,3-DIHYDROXY-1,4-DITHIOBUTANE	non-polymer		154.3	2	Chemie (DTT)
6	D, B	CITRATE ANION	non-polymer		189.1	2	Chemie (FLC)
7		water	water		18.0	71	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 308 (UniProt: Q7L266)

PDB	External Database	Details
Gly 1	-	linker
His 154	-	expression tag
His 154	-	expression tag
His 154	-	expression tag
His 154	-	expression tag
His 154	-	expression tag
His 154	-	expression tag
Met 154	-	SEE REMARK 999
Gly 309	-	linker
Ala 310	-	linker
Gly 311	-	linker
Ser 312	-	linker
Gly 313	-	linker
Ala 314	-	linker
Gly 315	-	linker
Ser 316	-	linker
Gly 317	-	linker
Ala 318	-	linker
Gly 319	-	linker
Gly 320	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	135068.7
Non-Polymers*	Number of molecules	25
	Total formula weight	1929.2
All*	Total formula weight	136997.9

*Water molecules are not included.