4ZM9

Crystal structure of circularly permuted human asparaginase-like protein 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	104.308, 111.107, 122.517
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.740 - 2.510
R-factor	0.20569
Rwork	0.204
R-free	0.23331
RMSD bond length	0.015
RMSD bond angle	1.684
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.390
High resolution limit [Å]	2.350	2.350
Number of reflections	49346
<I/σ(I)>	10.38
Completeness [%]	99.6	99.9
Redundancy	5.1	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	297	24 % PEG4000, 4 mM DTT, 0.15 mM Sodium citrate, pH4.6 Microseeding